Microfluidic experimental board and double-sided cell culture method

Abstract

The invention provides a microfluidic experimental board and a double-sided cell culture method. The microfluidic experimental board comprises a body, at least one culture medium filling hole, at least one culture room, at least one cell culture beaker, at least one waste liquor drain hole, flow passages and a sealing film, wherein the culture medium filling holes, the culture rooms and the wasteliquor drain holes all penetrate through the body, the cell culture beakers are arranged in the culture rooms, bottom openings of the cell culture beakers are closed through semipermeable membranes, the flow passages communicate with the culture medium filling holes, the culture rooms and the waste liquor drain holes so as to convey culture media to the culture rooms and convey waste liquor to thewaste liquor drain holes for draining, the sealing film is arranged on the back of the body and covers the culture medium filling holes, the flow passages, the culture rooms and the waste liquor drain holes. According to the microfluidic experimental board provided by the invention, microfluidic liquid supply control and cell culture are integrated and standardized, all structural and processingproblems encountered during integration are solved, co-culture of a variety of cells can be achieved, and a shearing force stimulating function is integrated.

Description

technical field [0001] The invention belongs to the field of microfluidics and biomedicine, and relates to a microfluidics experiment plate and a double-sided cell culture method. Background technique [0002] Cell culture is widely used in medical real-time detection and biological and pharmaceutical research in colleges and universities. At present, most cell cultures are limited to monolayer culture of single cells, however, more and more data show that the biological performance of single cells in monolayer culture and cells in animal tissues is not the same. The way to make cells closer to the culture in animals is to co-cultivate multiple cells, and even build a three-dimensional structure of multilayer cells. The current academia will use a relatively complex structure to simulate the microenvironment of the organ, which is called an organ chip. Moreover, most organ chips in academia are temporarily designed and processed according to the needs of specific organ res...

AI Technical Summary

Problems solved by technology

Moreover, most organ chips in academia are temporarily designed and processed according to the needs of specific organ research, and do not have versatility and stability.

Images