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Application of recombinant protein hid2 in the preparation of drugs for the treatment of colitis

A recombinant protein, ulcerative colitis technology, applied in the direction of recombinant DNA technology, application, drug combination, etc., can solve the problem of no related reports on the effect of ulcerative colitis

Active Publication Date: 2022-08-05
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have reported that ID2 has a variety of important biological roles in promoting cell proliferation, inhibiting cell differentiation, angiogenesis and tumorigenesis, but the role of ID2 in ulcerative colitis has not been reported yet

Method used

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  • Application of recombinant protein hid2 in the preparation of drugs for the treatment of colitis
  • Application of recombinant protein hid2 in the preparation of drugs for the treatment of colitis
  • Application of recombinant protein hid2 in the preparation of drugs for the treatment of colitis

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Embodiment Construction

[0022] In order to make the objectives, technical solutions and advantages of the present invention more clear, the following examples will further describe the present invention in detail. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The reagents and materials used in the following examples are commercially available unless otherwise specified.

[0023] 1.1 Preparation of recombinant protein hID2

[0024] 1.1.1 Construction of recombinant expression plasmid pET-ID2

[0025] Escherichia coli strain E.coli.DH5α and Escherichia coli expression plasmid pET-30a(+) are products of Invitrogen Company kept in the laboratory. PCR primers were purchased from Shanghai Yingjun Biotechnology Co., Ltd., and corresponding restriction sites were introduced respectively.

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Abstract

The invention relates to a recombinant human ID2 (inhibitor of DNA binding 2) protein. The recombinant protein hID2 is prepared by constructing a human ID2 gene recombinant plasmid and efficiently expressing it in Escherichia coli. The recombinant protein hID2 has significant curative effect in the treatment of ulcerative colitis without adverse reactions and side effects.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a recombinant human ID2 (inhibitor of DNA binding2) protein, which is prepared by constructing a human ID2 gene recombinant plasmid and efficiently expressing it in Escherichia coli, and is used for preparing a drug for treating ulcerative colitis . technical background [0002] Ulcerative colitis (UC) is a chronic nonspecific intestinal disease of unknown etiology. The lesions are mainly located in the mucosal layer of the colon, often involving the rectum and distal colon, and can also spread throughout the colon with continuous diffusion. Sex distribution. The disease is seen at any age, and is more common in 25-40 years old. Except for a few patients with abrupt onset, most patients have a slow onset, and the severity of the disease varies. Abdominal pain, diarrhea, mucus stool, pus and blood stool, tenesmus are the main clinical symptoms, histopathological manifestations ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17C12N15/12C12N15/70C07K14/47A61P1/00A61P1/04A61P1/12A61P7/04A61P29/00
CPCA61K38/1709A61K9/0019C07K14/4703C12N15/70A61P1/00A61P29/00A61P1/12A61P7/04A61P1/04
Inventor 吴敏娜任杰钟根深齐劲松景雪倩
Owner XINXIANG MEDICAL UNIV