Separation and culture method of ovarian granular cells of northern Guizhou Ma goats

A Qianbei hemp sheep and granular cell technology, applied in the field of cell biology, can solve the problems of few cleaning times, no clear regulation of cleaning times, probability of cell contamination, etc., to improve accuracy, easy operation, and fewer dead cells. Effect
CN112322576AInactive Publication Date: 2021-02-05GUIZHOU UNIV
0 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUIZHOU UNIV
Publication Date
2021-02-05
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention discloses a separation and culture method of ovarian granular cells of northern Guizhou Ma goats. The separation and culture method of ovarian granular cells of northern GuizhouMa goats comprises the following steps: sacrificing adult multiparous purebred northern Guizhou Ma goats by neck bloodletting, taking out ovaries, flushing away bloodstain, bringing the treated ovaries back to a sterile room, performing soaking with alcohol, rinsing with alcohol, normal saline and sterilized PBS, puncturing follicles with a needle, releasing ovarian granular cells, performing rinsing with a 10% complete medium, after centrifuging, taking a precipitate, continuously rinsing the precipitate with a sterilized PBS buffer solution, adding a 10% complete medium, and peforming blowing, beating, uniformly mixing, and culturing in a constant-temperature incubator. The pure ovarian granular cells can be separated through the method and the method is easy and convenient to operate, stable, and reliable and has good repeatability.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of cell biology, in particular to a method for separating and culturing ovarian granulosa cells of Qianbei Ma sheep. Background technique

[0002] Qianbei Ma sheep is an excellent local goat breed in Guizhou. It has the advantages of tolerance to rough feeding, strong disease resistance, docile sex, good meat quality, good quality of lamb skin, and higher reproductive rate than other local breeds in Guizhou. It is an excellent material for breeding multi-lamb strains.

[0003] As the main functional cells of the ovary, granulosa cells play an important role in the whole development process from the generation of primordial follicles to the discharge of mature follicles. Granulosa cells can act as a medium to make up for the lack of oocyte uptake of small molecules through gap junctions. At the same time, the growth and differentiation of granulosa cells is the key to the initiation and growth of primordial follicles....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More