Separation and culture method of ovarian granular cells of northern Guizhou Ma goats
A Qianbei hemp sheep and granular cell technology, applied in the field of cell biology, can solve the problems of few cleaning times, no clear regulation of cleaning times, probability of cell contamination, etc., to improve accuracy, easy operation, and fewer dead cells. Effect
Inactive Publication Date: 2021-02-05
GUIZHOU UNIV
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Abstract
The present invention discloses a separation and culture method of ovarian granular cells of northern Guizhou Ma goats. The separation and culture method of ovarian granular cells of northern GuizhouMa goats comprises the following steps: sacrificing adult multiparous purebred northern Guizhou Ma goats by neck bloodletting, taking out ovaries, flushing away bloodstain, bringing the treated ovaries back to a sterile room, performing soaking with alcohol, rinsing with alcohol, normal saline and sterilized PBS, puncturing follicles with a needle, releasing ovarian granular cells, performing rinsing with a 10% complete medium, after centrifuging, taking a precipitate, continuously rinsing the precipitate with a sterilized PBS buffer solution, adding a 10% complete medium, and peforming blowing, beating, uniformly mixing, and culturing in a constant-temperature incubator. The pure ovarian granular cells can be separated through the method and the method is easy and convenient to operate, stable, and reliable and has good repeatability.
Application Domain
Cell dissociation methodsArtificial cell constructs +1
Technology Topic
Granular cellBiomedical engineering +10
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