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Construction method of novel coronavirus Raman spectrum data center

A technology of Raman spectroscopy and coronavirus, which is applied in the field of construction of the new coronavirus Raman spectroscopy data center, can solve the problems of detecting virus interference and affecting the reliability of detection results, etc.

Active Publication Date: 2021-02-05
LASER FUSION RES CENT CHINA ACAD OF ENG PHYSICS
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Problems solved by technology

The impact of these factors has brought great interference to the detection of viruses by SERS technology, which seriously affects the reliability of the detection results.
At the same time, the use of Raman spectroscopy to detect viruses is still in the stage of laboratory research. At present, there is still no effective method for establishing a virus Raman spectroscopy database at home and abroad.

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  • Construction method of novel coronavirus Raman spectrum data center

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Embodiment 1

[0098] Such as figure 1 As shown, this embodiment provides a method for constructing a new coronavirus Raman spectrum data center, including the following steps:

[0099] S1. Constructing a Raman spectrum database of novel coronavirus structural proteins, said structural proteins including spike protein and nucleoprotein;

[0100] S2. Constructing a Raman spectrum database of novel coronavirus nucleic acid;

[0101] S3. Construct the Raman spectrum database of the new coronavirus particles;

[0102] S4. Constructing a Raman spectrum database of clinical testing samples for the new coronavirus;

[0103] The new coronavirus Raman spectrum database constructed in steps S1-S4 is stored in the new coronavirus Raman spectrum detection server to form a new coronavirus Raman spectrum data center.

[0104] Specifically, the step S1 includes the following sub-steps:

[0105] S1.1. Obtain the Raman spectrum of the purified structural protein of the new coronavirus;

[0106] S1.2. Ob...

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Abstract

The invention discloses a construction method of a novel coronavirus Raman spectrum data center. The method comprises the following steps: S1, constructing a novel coronavirus structural protein Ramanspectrum database; S2, constructing a novel coronavirus nucleic acid Raman spectrum database; S3, constructing a novel coronavirus particle Raman spectrum database; S4, constructing a Raman spectrumdatabase of a novel coronavirus clinical detection sample; and storing each novel coronavirus Raman spectrum database into a novel coronavirus Raman spectrum detection server to form a novel coronavirus Raman spectrum data center. According to the invention, a set of complete novel coronavirus Raman spectrum database is effectively established, reliable standard data support is provided for the novel coronavirus Raman detection technology, and the accuracy and confidence of a detection result are effectively improved.

Description

technical field [0001] The invention belongs to the field of novel coronavirus detection, and in particular relates to a method for constructing a novel coronavirus Raman spectrum data center. Background technique [0002] At present, the detection of the new coronavirus (SARS-CoV-2) is mainly based on the real-time fluorescent polymerase chain reaction (RT-PCR) nucleic acid detection method. There are many experimental steps, and the whole detection process takes about 4 hours. screening. [0003] Surface-enhanced Raman spectroscopy (SERS) technology can identify and detect specific virus molecules quickly, accurately, and in real time. However, the Raman spectra of biomacromolecules will be affected by many factors, such as biological activity, the complexity of physiological environment, the variability among individuals, and the interaction between biomacromolecules and SERS-enhanced substrates, etc. The impact of these factors has brought great interference to the det...

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Application Information

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IPC IPC(8): G16B50/30G01N21/65
CPCG01N21/658G16B50/30
Inventor 黄景林赵宗清周民杰杜凯乐玮倪爽陈果温家星李赜宇何智兵李波魏来曾勇祁道健赵松楠
Owner LASER FUSION RES CENT CHINA ACAD OF ENG PHYSICS
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