Aspergillus welwitschiae and application thereof

A technology of Aspergillus orchid and JX-S3, which is applied to Aspergillus orchid and its application field to achieve the effects of improving soil available phosphorus content and promoting growth and development

Active Publication Date: 2021-02-09
JIANGXI AGRICULTURAL UNIVERSITY
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  • Claims
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  • Aspergillus welwitschiae and application thereof
  • Aspergillus welwitschiae and application thereof
  • Aspergillus welwitschiae and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A high-efficiency phosphorus-solubilizing fungus JX-S3 strain was screened from the rhizosphere soil of Chinese fir plantation in Shixi Forest Farm, Taihe County, Ji'an City, Jiangxi Province.

[0029] The main morphology and biological characteristics of the JX-S3 strain: cultivated on the PDA medium plate for 7 days, the bacterium colony is dark brown ( figure 1 ); conidiophores, apical capsules, stalks and conidia synthetic spore heads, conidia spherical or subspherical ( figure 2 ).

[0030] image 3 It is the phosphate solubilizing circle produced by the strain JX-S3 cultured on NBRIP medium for 7 days, indicating that JX-S3 has the ability to solubilize phosphorus and is a phosphate solubilizing bacterium.

[0031] Molecular Biology Identification:

[0032] Extract the DNA of the JX-S3 strain, use the corresponding primers to PCR amplify to obtain its ITS, BenA and CaM fragments, and then sequence the genes. The sequence of the ITS fragment is shown in SEQ ID ...

Embodiment 2

[0034] Embodiment 2: Determination of the biomass of Aspergillus 100-year-old Aspergillus JX-S3 under different initial pH

[0035]Inoculate the activated JX-S3 strain in PDA liquid medium (potato 200g, glucose 20g, distilled water 1000mL), 25°C, 120r / min shaking culture for 5d as seed liquid, set the pH of PDA liquid medium to 1.5, 2.5 , 3.5, 4.5, 5.5, 6.5 six gradients, divided into 250ml Erlenmeyer flasks, the filling volume is 100mL. Subsequently, the seed solution was respectively inoculated into the PDA liquid medium according to the inoculum amount of 1% (v / v), and each treatment was repeated three times, and the culture was shaken at 25° C. and 120 r / min for 8 days. After the mycelia were filtered, they were dried at 65°C for 48 hours and weighed. The results are shown in Table 1. The JX-S3 bacterial strain grows better under the different acidic conditions of pH 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, and there is no difference in the mycelial biomass. The above shows that ...

Embodiment 3

[0038] Embodiment 3: The test of dissolving ability of Aspergillus 100-year-old Aspergillus JX-S3 to different phosphorus sources

[0039] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, mix well and sterilize.

[0040] Phospholytic medium B: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly and sterilized.

[0041] Phospholytic medium C: ferric phosphate (FePO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly and sterilized.

[0042] Phosphate-dissolving medium D: calcium hydrogen phosphate (CaHPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly a...

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Abstract

The invention discloses Aspergillus welwitschiae and application thereof. The preservation number of the Aspergillus welwitschiae JX-S3 disclosed by the invention is CCTCC (China Center For Type Culture Collection) NO: M 2020217; the Aspergillus welwitschiae is obtained by screening from rhizosphere soil of cunninghamia lanceolata and has and has efficient phosphate solubilizing capacity; the Aspergillus welwitschiae has better dissolving capability on five kinds of insoluble phosphates including calcium phosphate (Ca3(PO4)2), ferric phosphate (FePO4), calcium hydrophosphate (CaHPO4), aluminumphosphate (AlPO4) and calcium phytate (C6H6Ca6O24P6); and a liquid inoculant prepared from the strain has a remarkable growth promoting effect on the ground diameter and seedling height of the cunninghamia lanceolata. Therefore, an excellent strain resource is provided for developing an environment-friendly biological special fertilizer for the cunninghamia lanceolata.

Description

technical field [0001] The invention belongs to the field of microbes and biological fertilizers, and in particular relates to a centenarian aspergillus aspergillus and its application. Background technique [0002] Chinese fir (Cunninghamia lanceolata) is the main timber tree species in southern my country. It has the characteristics of wide planting area, large stock volume and high yield. It plays an important role in forest carbon sequestration, economic and ecological construction. However, in recent years, my country's Chinese fir plantation is facing the status quo of low productivity, insufficient supply of forest land nutrients, and the decline of soil fertility have seriously affected the resource cultivation of Chinese fir plantation, threatening the sustainable development of Chinese fir plantation. [0003] Rhizosphere microorganisms are key factors that mediate soil ecological functions and participate in the regulation of plant growth and development. Rhizosph...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05G3/00C05G5/10C05G5/12C05G5/27A01N63/34A01N43/38A01P21/00C12R1/66
CPCC05G3/00C05G5/10C05G5/12C05G5/27A01N63/34A01N43/38C12R2001/66C12N1/145
Inventor 张扬杨豆陈伏生毛瑢张林平万松泽张芸方向民
Owner JIANGXI AGRICULTURAL UNIVERSITY
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