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Sterilization fermentation tank for preparing cell culture medium

A fermenter and culture medium technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, sterilization method, etc., can solve uneven sterilization, expand contact area, kill bacteria and other problems, to achieve the effect of balancing the sterilization effect and increasing the contact area

Inactive Publication Date: 2021-02-12
王兆晶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, the inventors found that the existing sterilized fermenter mainly has the following defects: because the steam generated by the fermenter will continue to float on the upper end of the cell reagent when the fermenter is heated, and the fermenter cannot increase the flow rate of the steam Expand the contact area with steam and integrate into the cell reagent, thus increasing the sterilization time of the steam on the cell reagent and the cell reagent will form uneven sterilization, resulting in the inability of the bacteria in the cell reagent to be completely killed

Method used

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  • Sterilization fermentation tank for preparing cell culture medium
  • Sterilization fermentation tank for preparing cell culture medium
  • Sterilization fermentation tank for preparing cell culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Figure 1 to Figure 5 Shown:

[0025] The invention provides a sterilized fermenter for preparing cell culture medium,

[0026] Its structure includes a bracket 1, a controller 2, a feed port 3, a partition plate 4, and a fermentation tank 5. The bracket 1 is fixed on the lower end of the fermentation tank 5, and the controller 2 is installed on the outer ring of the fermentation tank 5. The feed inlet 3 and the partition plate 4 are located at the upper end of the partition plate 4 and are in communication state, and the upper end of the fermenter 5 is connected to and communicated with the partition plate 4 .

[0027] The interior of the fermenter 5 is provided with a sealing ring 51, a stirring mechanism 52, an activity space 53, and an air suction barrel 54. The middle part of the sealing ring 51 is embedded with a stirring mechanism 52, and the activity space 53 is located on the outer ring of the stirring mechanism 52. position, the suction barrel 54 is installe...

Embodiment 2

[0034] Figure 6 to Figure 7 Shown:

[0035] The invention provides a sterilized fermenter for preparing cell culture medium,

[0036]Its structure comprises that described suction bucket 54 is provided with adsorption shaft 541, extractor 542, arc plate 543, conduit 545, and described adsorption shaft 541 is installed on the top of extractor 542, and described arc plate 543 and adsorption shaft 541 One body and below the adsorption shaft 541, the conduit 545 runs through the arc-shaped plate 543 to connect with the extractor 542, the middle part of the arc-shaped plate 543 runs through the conduit 545, and the arc-shaped plate 543 passes through its own shape It can effectively ensure the smooth operation of other components, and the conduit 545 can block the cell reagents through other components.

[0037] The conduit 545 is provided with a solid layer b11, an extension port b12, a rotating groove b13, a push block b14, and an exhaust plate b15. The solid layer b11 and the...

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Abstract

The invention provides a sterilization fermentation tank for preparing a cell culture medium. The sterilization fermentation tank structurally comprises a bracket, a controller, a feeding hole, a partition plate and a fermentation tank, the bracket is fixed at the lower end of the fermentation tank, the controller is arranged at the outer ring part of the fermentation tank, and the feeding hole and the partition plate are positioned at the upper end of the partition plate and are communicated with each other; the upper end of the fermentation tank is connected and communicated with the partition plate; the stirring mechanism arranged in the fermentation tank can stir a cell reagent and steam in the fermentation tank, so that a scraper passing through a sliding block can drive a driving device, and under the action of a spring, the scraper and the spring can continuously extend out and contract, so that the cell reagent in the fermentation tank can be uniformly stirred. The sterilization fermentation tank is advantaged in that the steam in the fermentation tank can be effectively pushed, so the contact area of the steam and a cell reagent is increased, and the steam and the cell reagent are mixed to achieve a rapid sterilization effect.

Description

technical field [0001] The invention relates to the field of sterilization of culture medium, in particular to a sterilized fermenter for preparing cell culture medium. Background technique [0002] Cell culture is to put the prepared cells and reagents into the fermenter step by step, so that the cells can be mixed with the corresponding reagents in the fermenter, and at the same time, the steam emitted by the fermenter can be heated by the inside of the fermenter. The reagent is sterilized, and the cell reagent can form a sterile state after being sterilized by steam, and then fermented in the closed space of the fermenter; [0003] In summary, the inventors found that the existing sterilized fermenter mainly has the following defects: because the steam generated by the fermenter will continue to float on the upper end of the cell reagent when the fermenter is heated, and the fermenter cannot increase the flow rate of the steam Expand the contact area with the steam and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/12
CPCC12M27/02C12M37/00
Inventor 王兆晶
Owner 王兆晶