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Triple efficient ammonia nitrogen degradation microecological preparation and preparation method thereof

A kind of micro-ecological preparation and high-efficiency technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unfavorable human health, achieve maintenance of self-repair ability, eliminate rebound phenomenon of nitrite content, preserve The long-term effect

Pending Publication Date: 2021-02-23
南京师盛生态环境研究院有限公司
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Problems solved by technology

The impact on human health The ammonia nitrogen in the water can be converted into nitrite under certain conditions. If you drink it for a long time, the nitrite in the water will combine with protein to form nitrosamine, which is a strong carcinogen and extremely harmful to human health.

Method used

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  • Triple efficient ammonia nitrogen degradation microecological preparation and preparation method thereof

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preparation example Construction

[0025] A preparation method of a triple efficient ammonia nitrogen degrading micro-ecological preparation, comprising the following preparation steps:

[0026] Step 1: Weigh 1g of the sludge sample from the aerobic aeration tank, shake it in 100ml sterile PBS buffer solution, bathe in 90°C water bath for 10min, take 0.1ml and spread it on the manganese salt nutrient agar plate, incubate at 28°C for 48h, take Bacteria with large colonies were purified and re-cultivated. After spore staining and Gram staining, the antagonistic indicator bacteria were cultured in nutrient broth for 18 hours, and 0.1ml was spread on the nutrient broth agar plate, and a hole punch with an inner diameter of 8mm was used. Drill 1 hole on the plate, inject 0.1ml of Bacillus culture solution into the hole, culture in a 28°C incubator for 24 hours, then transfer to Bacillus licheniformis liquid medium;

[0027] Step 2: Take 8g of the river bottom sludge sample, add 100ml of sterilized seawater, place it...

Embodiment 1

[0036] A triple high-efficiency ammonia nitrogen degradation micro-ecological preparation, which is prepared by mixing Bacillus licheniformis, Rhodobacter sphaeroides and lactic acid bacteria in proportion, and the ratio of Rhodobacter sphaeroides, Bacillus licheniformis and Lactobacillus plantarum is 3:1:1.

Embodiment 2

[0038] A triple high-efficiency ammonia nitrogen degradation micro-ecological preparation, which is prepared by mixing Bacillus licheniformis, Rhodobacter sphaeroides and lactic acid bacteria in proportion, and the ratio of Rhodobacter sphaeroides, Bacillus licheniformis and Lactobacillus plantarum is 2:1:1.

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Abstract

The invention discloses a triple efficient ammonia nitrogen degradation microecological preparation which is prepared by mixing bacillus licheniformis, rhodobacter sphaeroides and lactic acid bacteriain proportion. The invention further discloses a preparation method of the triple efficient ammonia nitrogen degradation microecological preparation. The preparation method comprises the following preparation steps: step 1, separation, screening and culture of the bacillus licheniformis; step 2, treatment of a sludge sample; step 3, enrichment culture; step 4, separation, screening and culture ofthe rhodobacter sphaeroides; step 5, separation, screening and culture of the lactobacillus plantarum; step 6, activation; and step 7, proportional mixing. The triple efficient ammonia nitrogen degradation microecological preparation has the beneficial effects that bacillus, lactic acid bacteria and photosynthetic bacteria are organically combined from the perspective of biological mutualism, thesynergistic effect of microorganisms is fully exerted, the defects of a single strain are overcome, the concentrations of ammonia nitrogen and nitrite nitrogen in aquaculture water bodies are rapidlyand efficiently reduced without rebounding, and the preparation is harmless to human bodies, does not pollute the environment and is long in storage period.

Description

technical field [0001] The invention specifically relates to a preparation method of a triple high-efficiency ammonia nitrogen degrading micro-ecological preparation. Background technique [0002] Nowadays, due to the impact of human activities, the ammonia nitrogen indicators in urban rivers and relatively open waters remain high, and the black and odor problem has seriously affected the urban environment. The ecological environment problems caused by this have already affected the human home environment and the image of the city. Ammonia nitrogen refers to the free ammonia (NH 3 ) and ammonium ions (NH 4+ ) in the form of nitrogen. Therefore, when the content of ammonia nitrogen in water increases, it refers to the compound nitrogen existing in the form of ammonia or ammonium ions. Nitrate nitrogen (NO 3 -N) mainly, free ammonia (NH 3 ) and ammonium ions (NH 4+ ) form of nitrogen in polluted water is called hydrated ammonia, also known as non-ionized ammonia. Non-i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/10C12R1/01C12R1/25
CPCC02F3/02C02F3/34C12N1/20Y02W10/10
Inventor 王凯曹惠忠
Owner 南京师盛生态环境研究院有限公司
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