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Application of FXYD3 as gastric cancer diagnosis marker and treatment target

A diagnostic marker and marker technology, applied in the field of medical biological detection, can solve the problems of few reports and unclear effects, and achieve the effects of inhibiting the formation of intratumoral blood vessels and enhancing the sensitivity of chemotherapeutic drugs

Active Publication Date: 2021-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of FXYD3 in the development of gastric cancer is still unclear, and there are few reports

Method used

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  • Application of FXYD3 as gastric cancer diagnosis marker and treatment target
  • Application of FXYD3 as gastric cancer diagnosis marker and treatment target
  • Application of FXYD3 as gastric cancer diagnosis marker and treatment target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] Gastric cancer tissues and adjacent normal tissues were obtained from patients who came to the hospital for surgical treatment; gastric cancer cell lines AGS, BGC-823, MGC-803, MKN-45 and normal gastric epithelial cell line EGS1 were all treated with DMEM containing 10% FBS (high Sugar) medium, cultured at 37°C, 5% CO2 and 95% humidity; BALB / c nude mice, 5 weeks old, were raised in an SPF environment.

[0053] (1) qPCR

[0054] Tissue and cell samples were collected, total RNA was extracted, and reverse transcribed into cDNA. The primer sequences of the designed and synthesized FXYD3 gene and the internal reference GAPDH gene are as follows:

[0055] FXYD3-F:5'-GCCCAAAGCTGATGAGGACAGAC-3',

[0056] FXYD3-R:5'-GAGTCCCCAGCAGAGGAGGAAGAA-3';

[0057] GAPDH-F: 5'-ATGACATCAAGAAGGTGGTGAAGCAGG-3',

[0058] GAPDH-R: 5'-GCGTCAAAGGTGGAGGAGTGGGT-3'.

[0059] The amplification conditions are as follows: 95°C for 1min; 95°C for 10s, 60°C for 30s, 40cycles. Sequence Detectionsoft...

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Abstract

The invention discloses application of FXYD3 as a gastric cancer diagnosis marker and a treatment target, and relates to the technical field of medical biological detection. According to the invention, a specific detection primer is constructed, which can be used for gastric cancer diagnostic reagent or kit, and can be used for detecting tissue specimens or blood samples such as blood plasma, blood serum and blood platelets. The invention also comprises application of the FXYD3 in preparation of a pharmaceutical composition for preventing or treating gastric cancer, and the drug can inhibit expression of the FXYD3. The FXYD3 has an important function in the occurrence and progress of gastric cancer, which can be used as a target for diagnosis and treatment, and has a good clinical application value.

Description

technical field [0001] The invention belongs to the technical field of medical biological detection, and in particular relates to the application of FXYD3 as a diagnostic marker and therapeutic target of gastric cancer. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive tract. Although its incidence is generally declining globally, it still maintains a high incidence in China, Japan, and Latin America. Since most patients with gastric cancer are already at an advanced stage when they are diagnosed, the timing of surgery is lost, which seriously affects the quality of life of patients and the prognosis is extremely poor. The prognosis of gastric cancer is closely related to early diagnosis, and elucidating specific molecular markers in the progression of gastric cancer is crucial to understanding the molecular mechanism of gastric cancer progression and providing possible therapeutic targets. Studies have shown that the discovered early...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/06A61K33/243A61P35/00
CPCC12Q1/6886A61K45/06A61K33/243A61P35/00C12Q2600/158C12Q2600/166C12Q2600/136C12Q2600/106C12Q2600/118A61K2300/00
Inventor 李启杰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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