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Method for identifying and evaluating aphid resistance grades of alfalfa varieties

An evaluation method, alfalfa technology, applied in horticultural methods, botanical equipment and methods, instruments, etc., can solve problems such as plant hazards, alfalfa death, and affecting normal production of alfalfa, and achieve the effect of reducing planting costs and ensuring yield and quality

Pending Publication Date: 2021-03-02
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pests live by eating crops, which will cause serious damage to plants (such as alfalfa), seriously affect the normal production of alfalfa, greatly reduce the yield of alfalfa and even lead to the death of alfalfa

Method used

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  • Method for identifying and evaluating aphid resistance grades of alfalfa varieties
  • Method for identifying and evaluating aphid resistance grades of alfalfa varieties
  • Method for identifying and evaluating aphid resistance grades of alfalfa varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Indoor aphid resistance identification and evaluation of alfalfa varieties: the natural infection method was used indoors, and aphid control experiments were carried out at the seedling stage of alfalfa varieties, and the indoor aphid resistance performance of alfalfa varieties was graded and evaluated.

[0040] Indoor aphid-resistant identification and evaluation of alfalfa varieties specifically includes the following steps: indoor alfalfa seedling cultivation, aphid inoculation and infestation of alfalfa seedlings, termination of aphid infestation of alfalfa seedlings, continued cultivation of alfalfa seedlings, indoor aphid-resistant grading and evaluation of alfalfa varieties.

[0041] Indoor aphid resistance identification and evaluation of alfalfa varieties, 12 alfalfa varieties including Crown, WL343HQ, Suntory, Debao, Plato, Forerunner, Surprise, Zhongmi 3, Gannong 4, Queen, MF4020, SR4030 were selected for indoor experiments.

[0042] Firstly: sow 12 alfalfa va...

Embodiment 2

[0054] In this example, field identification and evaluation of aphid resistance is carried out in the field, including the following steps: field test group design, field planting of alfalfa varieties, field insect resistance monitoring and recording, field aphid resistance performance classification and evaluation of alfalfa varieties.

[0055] In this example, field identification and evaluation of aphid resistance was carried out in the field. A total of 12 alfalfa varieties including Crown, WL343HQ, Suntory, Debao, Plato, Forerunner, Surprise, Zhonglu No. 3, Gannong No. 4, Queen, MF4020, and SR4030 were selected for indoor test.

[0056] Firstly, the 12 alfalfa varieties mentioned above were designed according to random blocks, and each variety was replicated 3 times, and planted in the experimental field of alfalfa main planting area. The sowing time is before July 15 every year, the average sowing rate is 1.5kg / mu, the area of ​​the plot is 5m×7.5m, and the field managem...

Embodiment 3

[0066] This example is a comprehensive evaluation of the aphid resistance results of the indoor alfalfa varieties and the aphid resistance results of the field alfalfa varieties in Examples 1 and 2, that is, the comprehensive evaluation of the alfalfa variety resistance, and the aphid resistance of the alfalfa varieties is obtained, as shown in the following table Shown:

[0067]

[0068] Conclusion: The indoor identification results are consistent with the field identification results. It takes a certain amount of time for the pests to infect the host and colonize. In the indoor identification, the aphids infested different alfalfa varieties for up to 28 days. The field identification method is a natural infection process, and the aphid colonization is stable. According to the comprehensive analysis of indoor and field identification results, among the 12 alfalfa varieties tested, Surprise, WL343HQ, and Debao are susceptible to aphids; , Suntory, Queen, SR4030 and MF4020 a...

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Abstract

The invention provides a method for identifying and evaluating aphid resistance grades of alfalfa varieties. The method comprises the steps of indoor aphid resistance identification and evaluation ofthe alfalfa varieties, field aphid resistance identification and evaluation of the alfalfa varieties, comprehensive evaluation of aphid resistance of the alfalfa varieties and aphid resistance mechanism research of the alfalfa varieties. Different methods are adopted indoors and in fields to research the aphid resistance of the alfalfa varieties, and then the two methods are comprehensively evaluated to obtain the aphid resistance grades of the different alfalfa varieties. When aphids eat alfalfa, an aphid resistance mechanism of the alfalfa varieties is analyzed by detecting external morphological characteristics, physiological and biochemical indexes and electrophysiological changes of the alfalfa varieties, reference can be provided for planting the alfalfa varieties in different geographical environments, the yield and quality of the alfalfa in regions are ensured, and the planting cost of the alfalfa is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of identification of plant insect resistance, in particular to a method for identification and evaluation of aphid resistance grades of alfalfa varieties. Background technique [0002] Alfalfa is a general term for plants of the genus Medicago, commonly known as broccoli, and is a perennial flowering plant, the most famous of which is Medicago sativa as pasture, which has a wide variety, high nutritional value and contains the most abundant vitamins K, has the effects of clearing the spleen and stomach, benefiting the large and small intestines, and reducing bladder stones. Besides eating, it is mainly used to make hay, silage, pasture, etc. [0003] If alfalfa encounters diseases and insect pests during the growth period, it must be controlled in time, otherwise the yield and quality will be affected. Pests live by gnawing crops, which can cause serious damage to plants (such as alfalfa), seriously affect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/40A01G7/00A01K67/033G06Q50/02
CPCA01G22/40A01G7/00A01K67/033G06Q50/02
Inventor 张蓉魏淑花王颖高立原朱猛蒙马建华刘畅
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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