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Preparation method and application of probiotic spore capsid nanomaterial and probiotic composition

A technology of nanomaterials and probiotics, applied in the field of pharmaceutical preparations, can solve problems such as reduction, low efficiency of intestinal colonization, and inactivation of activity

Active Publication Date: 2021-08-17
ZHENGZHOU UNIV
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Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application based on probiotic spore capsid nanomaterials and probiotic composition, which can effectively solve the probiotics of various gastric acid intolerance. Bacteria are inactivated or reduced in activity after being taken orally in the stomach, resulting in the problem of low colonization efficiency in the intestinal tract, as well as inhibiting the growth and proliferation of harmful bacteria, restoring and rebuilding the intestinal barrier, and regulating the intestinal flora, thereby realizing the treatment of colitis and Effective prevention and treatment of colitis-associated colon cancer

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  • Preparation method and application of probiotic spore capsid nanomaterial and probiotic composition

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Embodiment 1

[0019] In the specific implementation of the present invention, a preparation method based on probiotic spore capsid nanomaterial and probiotic composition comprises the following steps:

[0020] (1) Cultivation of probiotics: inoculate the lyophilized powder of probiotic Bacillus in the nutrient gravy liquid medium, place it in a constant temperature incubator at 37°C, and cultivate it at a shaking speed of 140r / min. During the cultivation process, add 0.005% MnSO by volume in nutrient broth broth 4 ·H 2 O, promote probiotics to produce spores, make them recover and grow and proliferate. When the bacteria proliferate to the logarithmic phase, collect the bacterial liquid and set it aside;

[0021] (2) Extraction of spores: Inoculate the bacterial solution prepared in step (1) into the nutrient gravy liquid medium according to the inoculation amount of 3% of the volume of the nutrient gravy liquid medium, and place it in a constant temperature incubator at 37°C. To continue ...

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Abstract

The present invention relates to a preparation method and application of a probiotic spore capsid nanomaterial and a probiotic composition, which can effectively solve the problem of inactivation or activity reduction of various gastric acid intolerance probiotics after being taken orally in the stomach, thereby causing colonization in the intestinal tract The problem of low efficiency, as well as the problem of inhibiting the growth and proliferation of harmful bacteria, restoring and rebuilding the intestinal barrier, regulating the intestinal flora, and then realizing the effective prevention and treatment of colitis and colitis-related colon cancer, first, cultivate probiotics, Make it produce spores, then extract the spore capsids through an extruder and prepare capsid nanomaterials (Capsid Nano‑materials, CNMs) with uniform particle size and stable properties, and then grow and cultivate the capsid nanomaterials and probiotics on them Substrate co-incubation to obtain spore capsid nanomaterials and probiotic composition; the preparation method of the invention is simple, the production cost is low and the gastric acid environment is stable, the intestinal flora is adjusted, the intestinal barrier is enhanced, and it is suitable for inflammatory bowel disease and inflammation-induced Cancer treatment is powered by new technologies.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, in particular to a preparation method and application of a probiotic spore capsid nanomaterial and a probiotic composition. Background technique [0002] In recent years, probiotic therapy has been widely used in the clinical treatment of various diseases due to its significant advantages in regulating the balance between gut microbes, the environment, and the immune system. It is difficult for most probiotics to survive in the extreme gastric acid environment after oral administration. Therefore, the number of live bacteria that can successfully reach the intestinal colonization and play a role cannot be guaranteed, which limits the use of most probiotics in oral drug delivery systems Applications. As a class of probiotics that can produce spores, Bacillus can not only promote intestinal digestion, but also be used to treat intestinal inflammation, regulate host symbiotic flora and in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C12R1/07C12R1/225C12R1/145
CPCA61K35/742A61K35/745A61K35/747A61K47/46A61P1/00A61P29/00A61P31/04A61P35/00C12N1/04C12N1/16C12N1/20
Inventor 王蕾宋庆龄张云张振中高慧王珂郑翠霞赵洪娟冯倩华张红岭
Owner ZHENGZHOU UNIV
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