Nucleic acid-drug-loaded nano material for improving tumor permeability through VEGF response and preparation method and application thereof

Abstract

The invention discloses a nucleic acid-drug-loaded nano material for improving tumor permeability through VEGF response. The nucleic acid-drug-loaded nano material comprises a drug-loaded nanoparticlecore and a nucleic acid shell, wherein the nucleic acid shell comprises DNA1, DNA2, DNA3 and DNA4, and the DNA3 contains a V7T1 aptamer for specifically recognizing VEGF protein. Due to that the DNA3contains the VEGF aptamer, the VEGF protein overexpressed in a tumor microenvironment can be specifically recognized, so that the DNA3 and the DNA4 fall off from nucleic acid-drug-loaded nanoparticles, and the DNA2 / DNA1 modified albumin drug-loaded nanoparticles are small in particle size and have good tumor permeability; and the whole nucleic acid-drug-loaded nano material is mainly composed ofnucleic acid and protein and has good biocompatibility. The invention further discloses a preparation method and application of the nucleic acid-drug-loaded nano material. The preparation method is simple in operation and low in cost.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a nucleic acid-albumin nanometer drug-loaded material capable of enhancing tumor permeability in response to VEGF, a preparation method and application thereof. Background technique [0002] Targeted drug delivery nanomaterials have been widely used in the treatment of solid tumors to improve the accuracy of cancer diagnosis and the effectiveness of treatment. In general, 50-100nm nanomaterials can prolong blood circulation and effectively gather and stay in tumor tissue through passive targeting (EPR effect). However, the solid tumor microenvironment also includes dense extracellular matrix, high cell packing density, increased interstitial flow pressure, and slow interstitial flow velocity. These factors make it difficult for large-sized nanomaterials to penetrate into deep tumor tissues, thereby significantly reducing the therapeutic effect of solid tumors. On the contrar...

AI Technical Summary

Problems solved by technology

These factors make it difficult for large-sized nanomaterials to penetrate into deep tumor tissues, thereby significantly reducing the therapeutic effect of solid tumors.

On the contrary, small-sized nanomaterials (≤20nm) can diffuse into the deep layer of the tumor, but are easily cleared by the liver and kidney, so they cannot effectively accumulate at the tumor site

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