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Anticoagulant polypeptide and application thereof

A technology of anticoagulation and blood coagulation, which is applied in the field of medicine and biology, and can solve unseen problems

Active Publication Date: 2021-03-09
HUBEI UNIVERSITY OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These results indicate that SjKI-1 has anti-inflammatory and anticoagulant effects, and it may play an important role in prolonging the survival of parasites in mammalian hosts, but there is no report on the study of Schistosoma haematobium Kunitz polypeptide

Method used

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  • Anticoagulant polypeptide and application thereof
  • Anticoagulant polypeptide and application thereof
  • Anticoagulant polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the screening of anticoagulant polypeptide

[0039] 1. Preparation of polypeptides

[0040] Using bioinformatics analysis to find the Kunitz sequence of Schistosoma haematobium, analyze these sequences, select the Kunitz sequence with complete structure, use the Signal P3.1 server to predict its signal peptide cleavage site, and name the target sequence as shp. Using pET28a as the expression vector, a recombinant plasmid containing the target sequence was constructed.

[0041] Six Schistosoma haematobium Kunitz sequences were named shp1, shp2, shp3, shp4, shp5 and shp6. According to the conserved sequence and three-dimensional modeling of the Kunitz polypeptide, the six cysteines in the sequence form three pairs of disulfide bonds in the way of CI~CVI, CIII~CV, and CII~CIV ( figure 1 )

[0042] in,

[0043] The nucleotide sequence of shp4 is shown in SEQ ID NO.1 (the TGA at the end is a stop codon)

[0044] SEQ ID NO.1:

[0045] TTTCGCAAAGGCAGCAGCGTG...

Embodiment 2

[0058] Embodiment 2, detection of target blood coagulation factor activity

[0059] In this example, the inhibitory effect of the anticoagulant active polypeptide shp4 on coagulation factors FXIa and XIIa was detected, and the residual activity of coagulation factors after incubation with the polypeptide was detected by substrate luminescence method.

[0060] 1. Detection of coagulation factor Xia (FXIa)

[0061] (1) Dilute each recombinant polypeptide prepared in Example 1 into 10 μM, 5 μM, 2.5 μM, 1.25μM, 0.625μM, 0.3125μM, 0 total seven concentration gradients, and then use coagulation factor buffer A to mix Ⅺa (Ⅺa was purchased from Haematologic Technologies Inc, product number: HCⅪA-0160) and the corresponding substrate (substrate of Ⅺa The substance is S2366, which was purchased from Chromogenix, product number 82109039), and diluted to 1.5 μg / mL and 1200 μM, respectively.

[0062] (2) Add 50 μl Ⅺa and 50 μl peptides of different concentrations to the wells of the 96-w...

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Abstract

The invention relates to anticoagulant polypeptide and an application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO.2. Anticoagulation is an endogenous blood coagulation resisting pathway, and the endogenous blood coagulation pathway is a blood coagulation effect participated or mediated by a blood coagulation factor XIa (FXIa). The invention also relates to a nucleotide sequence for encoding the anticoagulant polypeptide, and the nucleotide sequence is shown as SEQ ID NO.1. The invention also relates to an application of the anticoagulant polypeptide.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to an anticoagulant polypeptide and its application. Background technique [0002] According to the statistics of the World Health Organization, 15 million people die from thromboembolic diseases every year in the world. In my country, 10 million people get sick every year, and the number of deaths is 1 million. Thromboembolic diseases are characterized by high morbidity, high mortality and high disability rate. [1] . Common thrombotic diseases include acute coronary syndrome, ischemic stroke, and peripheral vascular disease, which seriously endanger human health. The treatment of thromboembolic diseases is divided into anticoagulant therapy, thrombolytic therapy and surgical thrombectomy, among which anticoagulant therapy is the mainstream treatment of thromboembolic diseases [2] . Common clinical anticoagulants are divided into traditional anticoagulan...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A61K38/17A61P7/02
CPCC07K14/43559A61P7/02A61K38/00Y02A50/30
Inventor 陈宗运丁莉罗旭东叶祥东朱雯
Owner HUBEI UNIVERSITY OF MEDICINE
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