Method for identifying production places of dendrobium officinale
A technology of Dendrobium officinale and origin, applied in the field of identification of the origin of Dendrobium officinale, can solve problems such as poor discrimination of medicinal materials, and achieve the effects of simple operation and objective standard of methods
Pending Publication Date: 2021-03-09
SHANGHAI UNIV OF T C M
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[0006] The purpose of the present invention is to overcome the disadvantages of poor identification of introduced and cultivated medicinal materials in the prior art, and provide a method for identifying the origin of traditional Chinese medicinal materials with high identification
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[0094] The specific implementation manners of the present invention will be further elaborated below.
[0095] The experimental materials, reagents and consumables and instruments and equipment used in the specific embodiment are as follows:
[0096] Experimental materials: fresh and dry products of Dendrobium candidum produced in Yunnan and Zhejiang (only for the first time), commercial products of Dendrobium candidum to be identified for origin, ITS and GAPDH primers, and molecular tags for origin (see Table 1 and Table 2);
[0097]Reagents and consumables: absolute ethanol (analytical grade), plant genomic DNA extraction kit DC104 (Nanjing Novizym Biotechnology Co., Ltd.), gel recovery kit DC301 (Nanjing Novizym Biotechnology Co., Ltd.), liquid nitrogen, agar Sugar, 50×TAE, RS138 nucleic acid dye, 6×DNA Loading Buffer, DL2000 DNA Ladder, phosphate buffer, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, mercaptoethanol, polyvinylpyrrolidone (pvp-40), sodium h...
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The invention discloses a method for identifying production places of dendrobium officinale, which comprises the following steps of: determining a group of fungi capable of representing structural regional differences in endophytic fungi communities of the dendrobium officinale by analyzing various dendrobium officinale samples with known production places, and designing production place moleculartags aiming at the group of fungi; obtaining dendrobium officinale samples with determined production places, and sequentially performing testing to obtain relative expression quantities of the samples corresponding to molecular tags of all the production places; constructing a database for reflecting the relationship between the dendrobium officinale production place and the relative expressionquantity of the molecular tags of the production place; testing the relative expression quantity of the dendrobium officinale samples of which the producing areas are to be determined corresponding toall producing area molecular tags; and comparing and verifying the measured relative expression quantities of the molecular tags of all the production places corresponding to the dendrobium officinale samples with data in a database, and determining the production places to which the dendrobium officinale samples belong. According to the method disclosed by the invention, according to the characteristic that a fungal community structure has an obvious regional difference, producing area molecular tags are constructed for producing area indicating bacterium groups with an indicating effect byscreening, and a method for judging and verifying is determined.
Description
technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and relates to an identification method for the origin of dendrobium officinale. Background technique [0002] Endophyte refers to a class of microorganisms that survive part or all of their life cycle inside healthy plant tissues without causing the host plant to show obvious symptoms of infection. Endophytes can also be understood as normal flora in plant tissues, including endophytes, bacteria, actinomycetes, etc. Among them, endophytes are the most studied. Endophytic fungi widely exist in healthy plant tissues. They are closely related to plants and are part of the plant micro-ecosystem. Endophytic fungi communicate with the tissue cells and metabolites of host plants in terms of material, energy and genes, forming a mutualistic symbiotic relationship in the long-term evolution process. [0003] In the course of long-term medical practice, ...
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IPC IPC(8): G16B25/10G06K9/62G01N33/00
CPCG16B25/10G01N33/0098G06F18/2135G06F18/23G06F18/22G06F18/24
Inventor 高林怡黎万奎王莉莉孙文静胡双
Owner SHANGHAI UNIV OF T C M