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Pinus sylvestris tissue culture medium and rooting method

A technique of tissue culture medium and tissue culture, which is applied in the field of plant cultivation, can solve the problems that restrict the large-scale popularization and application of Pinus sylvestris sylvestris breeding, the low efficiency of Pinus sylvestris, and the difficulty of rooting, so as to achieve great ecological and economic significance, reduce damage, The effect of promoting efficiency

Inactive Publication Date: 2021-03-12
苏州梵时轮园艺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the propagation of Pinus sylvestris has always been low in efficiency and difficult to take root. Among them, the efficiency of adventitious root induction in tissue culture is an important problem restricting the large-scale popularization and application of Pinus sylvestris breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Select Pinus sylvestris sylvestris needles as explants, and sterilize them:

[0028] Take young needle leaves as explants: Take young needle leaves in early spring in March.

[0029] The sterilization and disinfection treatment method is as follows: soak the young needles in an ultra-clean bench with 75% alcohol for 1 minute, wash them with sterile water for 3 times, and then wash them with 0.1% HgCl 2 After soaking for 10 minutes, soak with 5% chlorothalonil for 1 hour.

[0030] Step 2. Pretreatment of explants before induction:

[0031] After disinfection, soak the young needles in 10 g / L NaCl solution for 5 minutes. Stimulation by NaCl contributes to callus formation.

[0032] Step 3, preparation of callus induction culture:

[0033] The specific formula of callus induction medium is as follows: 1 / 2 LM medium + 2,4-D (0.4mg / L) + 6-BA (1.0 mg / L) + NAA (0.3mg / L) + yellow base salicylate Acid (0.2 μg / L) + β-mercaptoethanol (final concentration 1:2000) + Vor...

Embodiment 2

[0042] Step 1. Select Pinus sylvestris sylvestris needles as explants, and sterilize them: The steps are the same as in Example 1.

[0043] Step 2, pretreatment of explants before induction: the steps are the same as in Example 1.

[0044] Step 3, preparation of callus induction culture:

[0045] The specific formulation of the callus induction medium is as follows: 1 / 2 LM medium + 2,4-D (0.4mg / L) + 6-BA (1.5 mg / L) + NAA (0.5mg / L) + yellow base salicylate Acid (0.5 μg / L) + β-mercaptoethanol (final concentration 1:4000) + Vorinostat (SAHA, a histone deacetylase inhibitor, final concentration 0.9 μmol / L) + 5-azacytidine (5-AZA , a DNA methylase inhibitor 1.5 μmol / L).

[0046] Inoculate the explants treated in steps 1 and 2 into the culture medium. The needles became thicker 5 days after inoculation, and green granule callus appeared at the incision after 3 weeks.

[0047] Step 4, proliferation medium preparation and callus proliferation:

[0048] Proliferation medium compon...

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Abstract

The invention discloses a pinus sylvestris tissue culture medium and a rooting method. The rooting method comprises the following steps: selecting a tender needle leaf part of pinus sylvestris as an explant, and performing sterilization and disinfection treatment; soaking the explant into a NaCl solution for pretreatment before induction; inducing calluses; performing proliferation of the calluses; and performing rooting culture. By adopting the method for inducing and culturing the pinus sylvestris callus, the problems of high difficulty and low efficiency of an existing pinus sylvestris tissue culture technology are solved. The invention solves the problem of selection of tissue culture explants of pinus sylvestris plants, and provides a tissue culture method for induced differentiation,proliferation and rooting. The pinus sylvestris tissue culture medium and the rooting method are of great significance to breeding of pinus sylvestris and other coniferous plants, and have great ecological and economic significance.

Description

technical field [0001] The invention relates to a pine tissue culture medium and a rooting method, belonging to the technical field of plant cultivation. Background technique [0002] With its tall and straight shape and excellent wood texture, coniferous trees play an important role in the fields of solid wood, papermaking, and afforestation. Pinus sylvestris is one of the conifer species with important economic value and ecological function. However, the propagation of Pinus sylvestris has always been low in efficiency and difficult in rooting. Among them, the efficiency of adventitious root induction in tissue culture is an important problem restricting the large-scale popularization and application of Pinus sylvestris breeding. Contents of the invention [0003] The purpose of the present invention is to overcome the defects of the prior art, provide a kind of pine tissue culture medium and rooting method, to solve the difficult problem of plant callus induction and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 卢鹏
Owner 苏州梵时轮园艺科技有限公司
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