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Anti-cancer composition, combination product and preparation method and application of anti-cancer composition

A combined product and composition technology, applied in the field of medicine, can solve the problems of not being able to significantly improve the survival rate of patients with cancer indications, drug failure, drug resistance, etc., and achieve the effect of low cancer recurrence rate and convenient prevention and treatment of side effects

Inactive Publication Date: 2021-03-26
INST OF PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the course of olaparib chemotherapy, almost all patients developed drug resistance after a few months or more (average about 8.4 months), making the drug ineffective, and the blocked target protein has a significant chance to reverse mutant to wild-type protein
At present, olaparib can only delay the growth of cancer tissue and prolong the life of patients, but it cannot significantly improve the survival rate of patients with related cancer indications (like cisplatin for testicular cancer)

Method used

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  • Anti-cancer composition, combination product and preparation method and application of anti-cancer composition
  • Anti-cancer composition, combination product and preparation method and application of anti-cancer composition
  • Anti-cancer composition, combination product and preparation method and application of anti-cancer composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This example is used to illustrate the treatment results of the method of the present invention on different cancer cells.

[0055] (1) Drug dissolution: cisplatin and transplatinum powders are directly dissolved in sterile saline, and the concentration of cisplatin can easily reach 0.3-0.6mg / ml; Shake continuously in a water bath at ℃ for 30 min. The dissolved drug solution is filtered through a 0.22 μm sterile filter and used fresh or stored at -20 ℃ for use within one week.

[0056] (2) Drug treatment plan for cancer cells:

[0057] Simulate multiple dosing regimens in the clinical chemotherapy program (that is, use 3-4 times, and the interval between each dose is 3 to 4 weeks according to the growth of cancer cells. For cancer cells, select MDA-MB-436 breast cancer cells and Capan -1 Pancreatic cancer cells, the culture method is that the first three generations of cells after recovery completely follow the culture and passage methods of the commercial instructions...

Embodiment 2

[0065] This example is used to illustrate the safety experiment of the method of the present invention.

[0066] Through human normal primary cells and rat drug safety experiments, the drug concentration of the method of the present invention is within the low dose range (2-3μM) in clinical application, and does not cause any observable effects on human primary normal cells and rats. toxicity.

[0067] Specific steps: Human primary normal cells were purchased from the National Experimental Cell Resource Sharing Platform (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing) and ATCC (purchased by Beijing Zhongyuan Heju Economic and Trade Co., Ltd. in the United States), and cultured according to the method of purchasing the product manual , used within 6-8 passages. The drug treatment method is the same as the aforementioned "Cancer Drug Treatment Scheme". The concentration of the drug was selected in a series of doses that could cause cytotoxici...

Embodiment 3

[0072] This example is used to illustrate the effectiveness experiment of the method of the present invention - to overcome the formation of drug resistance.

[0073] Suitable for sensitive cancer cells As mentioned above, ~10,000 cancer cells (thawed in the same batch, low-passage cells p3-p5) were inoculated into 25cm 2 In the cell culture bottle, incubate and adhere to the wall in a CO2 incubator at 37°C for 2 hours, add the corresponding drug solution to the bottle to make the final concentration ~2×IC50, and then put the drug in the incubator for 2 hours (cisplatin, silk split mycin and the present invention, the method is as described above) or 7 days (olaparib, the method is as described above), and then washed with PBS, and then added fresh medium. After culturing for 3-5 weeks according to the cell growth rate, subculture the cells, and inoculate ~10,000 cancer cells / 25cm 2 Cell culture flasks, after treating the cells with gradually increasing drug concentration, ob...

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Abstract

The invention provides an anti-cancer composition. The anti-cancer composition comprises cisplatin and transplatin. The invention further provides a use method and application range of the anti-cancercomposition. According to the invention, the safety of combined application of the cisplatin and the transplatin to the interiors of animal bodies, the effectiveness of killing sensitive tumor cellsand the use method are comprehensively researched in detail. Under the conventional induction condition of drug resistance, the probability that the sensitive cells generate drug resistance is very low. In a parallel contrast experiment under the same condition, the probability that the cisplatin generates drug resistance is 87%, mitomycin-C and olaparib induce the drug resistance with high probabilities of higher than 90%, and the probability that the drug resistance is generated by the anti-cancer composition is 22%.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a combination method and application of an anticancer composition transplatinum and cisplatin. Background technique [0002] Cancer is currently the leading cause of human death in our country and the world. Genome instability and decreased ability to repair DNA damage are important causes in the development of cancer. Cisplatin (cisplatin) is one of the most widely used chemotherapeutic drugs in clinic. Except testicular cancer can be cured by cisplatin in 80-90% of patients without recurrence (known as the gold standard of cancer treatment), cisplatin in the treatment of other types of cancer, sooner or later drug resistance and cancer recurrence, Can not achieve the effect of cure. The basis of cisplatin as a chemotherapy drug is that it can hinge DNA, hinder DNA replication and transcription, and induce cancer cell death. Among them, cancer types with defects in DNA re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/243A61P35/00A61P35/02
CPCA61K33/243A61P35/00A61P35/02A61K2300/00
Inventor 王鹏业刘玉如
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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