Renal cell carcinoma biomarker and application thereof

A biomarker, renal cell carcinoma technology, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problem of molecular indicators to evaluate the prognosis of RCC patients

Pending Publication Date: 2021-03-26
GENERAL HOSPITAL OF NUCLEAR IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are currently no ideal molecular indicators to e

Method used

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  • Renal cell carcinoma biomarker and application thereof
  • Renal cell carcinoma biomarker and application thereof
  • Renal cell carcinoma biomarker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1. prepares the test kit (50 times of reaction) of predicting RCC patient's transfer and prognosis

[0053] 1. Trizol: 50ml;

[0054] 2. Chloroform: 20ml;

[0055] 3. Isopropanol: 30ml;

[0056] 4.75% ethanol; 60ml;

[0057] 5. DEPC water: 10ml;

[0058] 6. Oligo (dT) primer mixture: 100ul;

[0059] 7. 200U / ul M-MLV reverse transcriptase: 50ul;

[0060] 8. SYBR qPCR Mix: 500ul;

[0061] 9. 10uM target gene-specific primer (its sequence is shown in SEQ NO: 1-14): 100ul each;

[0062] 10. 10uM internal reference gene β-actin-specific primer (its sequence is shown in SEQ NO: 15-16): 100ul each.

Embodiment 2

[0063] Example 2. Detection and Analysis of Molecular Markers in Tissue Samples

[0064] 1. Collect the RCC tissues to be tested, normal paracancerous tissues, and distantly metastatic RCC tissues, wash them with normal saline, and freeze them in liquid nitrogen.

[0065]2. Tissue RNA extraction: Add liquid nitrogen into the mortar, cut the tissue into pieces and grind it in liquid nitrogen, take 100mg of tissue and add it to 1ml Trizol, and mix well. Leave it at room temperature for 5 minutes, add 200uL / tube of chloroform, shake vigorously and mix for 15 seconds. Centrifuge at 12,000 rpm for 15 minutes. Carefully pipette the upper aqueous phase into a new Ep tube, add an equal volume of isopropanol, invert and mix well, place at room temperature for 5 minutes, and centrifuge at 12,000 rpm for 10 minutes. Carefully discard the supernatant, add 75% ethanol, mix well and centrifuge at 8000 rpm for 8 minutes. Remove the supernatant, and add DEPC water to dissolve the RNA after...

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Abstract

The invention discloses a renal cell carcinoma biomarker. As a metastasis marker, the renal cell carcinoma biomarker is used for diagnosing and/or evaluating renal cell carcinoma prognosis and the risk of metastasis; or, as a specific molecular target of the renal cell carcinoma, the renal cell carcinoma biomarker is applied to treatment of the renal cell carcinoma; and the biomarker comprises oneor more of THBS2, SCGB1A1, NKX2-1, COL11A1, DCN, COL1A1 and SFTPB. By detecting the expression levels of THBS2, SCGB1A1, NKX2-1, COL11A1, DCN, COL1A1 and SFTPB in tissue of a renal cell carcinoma patient, whether the renal cell carcinoma patient has metastasis or not and prognosis can be judged, and the accuracy rate reaches 93% or more. Therefore, the disease progression, metastasis and prognosis of the renal cell carcinoma patient can be evaluated by detecting molecular biomarkers including the THBS2 and the like in the tissue of the renal cell carcinoma patient, and thus, symptomatic related treatment can be performed in time, the survival time and quality of the patient are respectively extended and improved; and the renal cell carcinoma biomarker has relatively great economic value and social benefits.

Description

technical field [0001] The invention relates to the technical field of biomedicine, more specifically, it relates to renal cell carcinoma biomarkers and applications thereof. Background technique [0002] Renal cell carcinoma (RCC) is a common cancer worldwide, accounting for about 2-3% of adult malignancies. In 2017, approximately 63,990 new cases of RCC and more than 14,400 kidney cancer-related deaths were reported in the United States. In China, the incidence of RCC is on the rise. In 2014, there were approximately 68,300 new RCC cases and 25,600 RCC-related deaths. Metastatic dissemination is the most important factor affecting the prognosis of RCC. The 5-year survival rate of patients with primary metastases is about 10%, and the 5-year survival rate of patients with non-metastatic and advanced metastases is 70% to 90%. When first diagnosed, nearly 60% of patients were localized cancer, and 20% were distant metastases. The metastatic spread of RCC mainly occurs in ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/118
Inventor 张永胜曹志飞涂健杨天宇钮慧沈珊珊
Owner GENERAL HOSPITAL OF NUCLEAR IND
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