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Cell assembly method and device based on medical B-ultrasonic machine

An assembly method and B-ultrasound machine technology are applied in tissue cell/virus culture devices, stress-stimulated microbial growth methods, biochemical cleaning devices, etc. Solve problems such as ultrasonic assembly and arrangement to achieve the effect of simple structure and easy operation

Active Publication Date: 2021-03-30
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]However, most of the traditional body ultrasonic cell assembly devices consist of two metal electrodes connected to a piezoelectric ceramic pressure transducer, and its working frequency usually only covers one frequency range of bandwidth, the impedance effect is strong outside this frequency range, and cells cannot be effectively assembled and arranged by ultrasound, and it is difficult to meet the needs of cell arrangement in tissue engineering and other biological research

Method used

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  • Cell assembly method and device based on medical B-ultrasonic machine
  • Cell assembly method and device based on medical B-ultrasonic machine
  • Cell assembly method and device based on medical B-ultrasonic machine

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Embodiment 1: Medical B-ultrasound linear array probe is used for assembly and arrangement of polystyrene microspheres

[0049] The material of the microspheres in the microsphere solution is polystyrene, the color is purple, the diameter is 10um, and the density is 1.05g / cm 3 , whose physical properties are similar to those of cells, the concentration of the microsphere solution is 1mg / ml. In this example, polystyrene microspheres were used to simulate cells.

[0050] Such as figure 1 As shown, the polystyrene microsphere cell assembly device of the embodiment of the present invention includes three parts: a suction cup universal vise 3 , a medical B-ultrasound machine 2 , and a cell assembly chamber 1 . The probe used in the medical B-ultrasound machine is a medical B-ultrasound linear array probe. The scanning method of medical B-ultrasound linear array probe is as follows: image 3 as shown in a.

[0051] The model of the suction cup universal vise 3 is MNT-60885...

Embodiment 2

[0058] Example 2: Medical B-ultrasonic phased array probes are used for assembly and arrangement of polystyrene microspheres

[0059] The material of the microspheres in the microsphere solution is polystyrene, the color is purple, the diameter is 10um, and the density is 1.05g / cm 3 , whose physical properties are similar to those of cells, the concentration of the microsphere solution is 1mg / ml. In this example, polystyrene microspheres were used to simulate cells.

[0060] Such as figure 1 As shown, the polystyrene microsphere cell assembly device of the embodiment of the present invention includes three parts: a suction cup universal vise 3 , a medical B-ultrasound machine 2 , and a cell assembly chamber 1 . The probe used in the medical B-ultrasound machine is a medical B-ultrasound phased array probe. The scanning method of medical B-ultrasound phased array probe is as follows: Figure 4 as shown in a.

[0061] The model of the suction cup universal vise 3 is Minate ...

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Abstract

The invention discloses a cell assembly method, which comprises the following steps: step 1, resuspending cells needing to be assembled and arranged by using a phosphate buffer solution, and injectingthe cells needing to be assembled and arranged into a cell assembly chamber; step 2, placing the cell assembly chamber right above a probe of the medical B-ultrasonic machine, and connecting the cellassembly chamber with an ultrasonic coupling agent; and step 3, starting the medical B-ultrasonic machine, adjusting the frequency to be 1-10MHz, the MI to be 0.6-1.4 and the TIS to be 0.6-1.2 on a control panel of the medical B-ultrasonic machine, keeping signals for 1-10min, and assembling and arranging cells. The linear arrangement or complex pattern arrangement of cells can be realized, whichis simple and easy to operate. The invention also provides an application of the medical B-ultrasonic machine in cell assembly.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering under the biomedical engineering, and relates to a cell assembly method and a device thereof based on a medical B-ultrasound machine. Background technique [0002] In the field of tissue engineering technology, the communication and function of many cells are based on certain shape and structure signals. Therefore, in order to achieve specific tissue functions and guide specific differentiation directions, it is necessary to assemble and arrange cells and other samples according to specific structures. In other words, The ordered assembly arrangement of cells plays an increasingly important role in the controlled growth of cells. [0003] However, most of the traditional cell arrangement methods use molds to passively arrange cell solutions. The disadvantage of this passive arrangement is that it can only assemble and arrange cell solutions, but cannot assemble and arrange ce...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/22C12M1/00C12M3/00C12N5/00
CPCC12M35/04C12M23/10C12M23/22C12N5/00C12N2521/10
Inventor 陈璞陈司晗徐晓冬
Owner WUHAN UNIV