Yak frozen semen diluent and preparation method thereof

A technology of freezing semen and diluent, applied in the field of bioengineering, can solve the problems of unevenness, high viscosity, unstable effect, etc., and achieve the effect of improving the survival rate, improving the quality, and being easy to popularize and apply

Active Publication Date: 2021-04-02
香格里拉藏龙生物开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, when freezing semen, it is necessary to add cryoprotectants, nutrients, salts, antibiotics and other components to the diluent. The most commonly used non-permeable cryoprotectant is egg yolk, such as patent applications 200510096117.5, 202010744351.9, etc. Although egg yolk has long-term use experience and better protective effects, but on the one hand, its organic components are complex, the effect is unstable and it is n

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method for yak frozen semen dilution, comprising the steps of:

[0029] Step (1), using double-distilled water to ultrasonically extract Cistanche at room temperature to obtain an extract of Cistanche; the mass ratio of double-distilled water to Cistanche is 100:3;

[0030] In step (2), crush Curculigo curculifolia until the particle size does not exceed 2mm, and then use double distilled water to extract the crushed Curculigo curculiculata at 50°C to obtain Curculigo curculigo extract; the mass ratio of double distilled water to Curculigo curculigo is 500: 5;

[0031] Step (3): Soak Gorgon fruit overnight, refine with double distilled water, filter, and adjust the filtrate to a density of 1.12±0.03g / cm with double distilled water 3 , to obtain Gorgon seed liquid;

[0032] Step (4), take the extract of Cistanche deserticola obtained in step (1), the extract of curculigo grass obtained in step (2), the Gorgon fruit liquid obtained in step (3), sodium citr...

Embodiment 2

[0036] A preparation method for yak frozen semen dilution, comprising the steps of:

[0037] Step (1), using double-distilled water to ultrasonically extract Cistanche at room temperature to obtain an extract of Cistanche; the mass ratio of double-distilled water to Cistanche is 100:1;

[0038] In step (2), crush Curculigo curculifolia until the particle size does not exceed 2mm, and then use double distilled water to extract the crushed Curculigo curculigo at 45°C to obtain Curculigo curculigo extract; the mass ratio of double distilled water to Curculigo curculigo is 500: 2;

[0039] Step (3): Soak Gorgon fruit overnight, refine with double distilled water, filter, and adjust the filtrate to a density of 1.12±0.03g / cm with double distilled water 3 , to obtain Gorgon seed liquid;

[0040] Step (4), take the extract of Cistanche deserticola obtained in step (1), the extract of curculigo grass obtained in step (2), the Gorgon fruit liquid obtained in step (3), sodium citrate,...

Embodiment 3

[0049] A preparation method for yak frozen semen dilution, comprising the steps of:

[0050] Step (1), using double-distilled water to ultrasonically extract Cistanche at room temperature to obtain an extract of Cistanche; the mass ratio of double-distilled water to Cistanche is 100:5;

[0051] Step (2), crush Curculigo curculiculi until the particle size does not exceed 2mm, and then extract the crushed Curculigo curculigo with double distilled water at 60°C to obtain Curculigo curculigo extract; the mass ratio of double distilled water to Curculigo curculigo is 500: 7;

[0052] Step (3): Soak Gorgon fruit overnight, refine with double distilled water, filter, and adjust the filtrate to a density of 1.12±0.03g / cm with double distilled water 3 , to obtain Gorgon seed liquid;

[0053] Step (4), take the extract of Cistanche deserticola obtained in step (1), the extract of curculigo grass obtained in step (2), the Gorgon fruit liquid obtained in step (3), sodium citrate, gluco...

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Abstract

The invention relates to a yak frozen semen diluent and a preparation method thereof, and belongs to the technical field of bioengineering. The frozen semen diluent is prepared from a herba cistancheextracting solution, a rhizoma curculiginis extracting solution, a semen euryales solution, sodium citrate, glucose, raffinose, resveratrol, tetrasodium glutamate diacetate, corn oligopeptide and thelike. The herba cistanche extracting solution, the rhizoma curculiginis extracting solution and the semen euryales liquid are introduced to maintain sperm activity, resveratrol is used as an antioxidant, tetrasodium glutamate diacetate is used as a chelating agent, raffinose is used for guaranteeing the cell membrane protection function, and the prepared yak frozen semen diluent can obviously improve the post-freezing motility rate and the acrosome integrity rate of sperms and improve the post-freezing quality; moreover, due to the fact that no animal-derived component exists, the risk of infection or diseases is avoided, and application and popularization are easy.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a yak frozen semen dilution and a preparation method thereof. Background technique [0002] Yak (scientific name: Bos mutus or Bos grunniens, English name: wild yak), belongs to Mammalia, Eutheria, Artiodactyla, Ruminantia, Bovidae, and Bovine subfamily animals. It is centered on the Qinghai-Tibet Plateau of China and its adjacent areas. One of the unique and rare cattle species in alpine and subalpine alpine regions, herbivorous ruminant livestock. The yak can adapt to the alpine climate and is the mammal that lives at the highest altitude (except humans) in the world. It is distributed in the Qinghai-Tibet Plateau of China at an altitude of more than 3,000 meters. Yak is called Yake in Tibetan, and it is commonly called "yak" in the world, which is the transliteration of Tibetan. Yaks sound like pigs, so they are also called pigs. Western countries are als...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0221
Inventor 陈树合
Owner 香格里拉藏龙生物开发股份有限公司
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