Yak frozen semen diluent and preparation method thereof

Abstract

The invention relates to a yak frozen semen diluent and a preparation method thereof, and belongs to the technical field of bioengineering. The frozen semen diluent is prepared from a herba cistancheextracting solution, a rhizoma curculiginis extracting solution, a semen euryales solution, sodium citrate, glucose, raffinose, resveratrol, tetrasodium glutamate diacetate, corn oligopeptide and thelike. The herba cistanche extracting solution, the rhizoma curculiginis extracting solution and the semen euryales liquid are introduced to maintain sperm activity, resveratrol is used as an antioxidant, tetrasodium glutamate diacetate is used as a chelating agent, raffinose is used for guaranteeing the cell membrane protection function, and the prepared yak frozen semen diluent can obviously improve the post-freezing motility rate and the acrosome integrity rate of sperms and improve the post-freezing quality; moreover, due to the fact that no animal-derived component exists, the risk of infection or diseases is avoided, and application and popularization are easy.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a yak frozen semen dilution and a preparation method thereof. Background technique [0002] Yak (scientific name: Bos mutus or Bos grunniens, English name: wild yak), belongs to Mammalia, Eutheria, Artiodactyla, Ruminantia, Bovidae, and Bovine subfamily animals. It is centered on the Qinghai-Tibet Plateau of China and its adjacent areas. One of the unique and rare cattle species in alpine and subalpine alpine regions, herbivorous ruminant livestock. The yak can adapt to the alpine climate and is the mammal that lives at the highest altitude (except humans) in the world. It is distributed in the Qinghai-Tibet Plateau of China at an altitude of more than 3,000 meters. Yak is called Yake in Tibetan, and it is commonly called "yak" in the world, which is the transliteration of Tibetan. Yaks sound like pigs, so they are also called pigs. Western countries are als...

AI Technical Summary

Problems solved by technology

[0004] At present, when freezing semen, it is necessary to add cryoprotectants, nutrients, salts, antibiotics and other components to the diluent. The most commonly used non-permeable cryoprotectant is egg yolk, such as patent applications 200510096117.5, 202010744351.9, etc. Although egg yolk has long-term use experience and better protective effects, but on the one hand, its organic components are complex, the effect is unstable and it is not easy to make commercial products, on the other hand, because it is an animal-derived component, there is a potential risk of disease transmission

[0005] At the same time, due to the presence of more lipids in the yolk, there are problems such as excessive viscosity, large and uneven egg yolk fat particles in the application, which affect the movement rate of sperm, and are not conducive to observation and operation after freezing and thawing.