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A kind of bioactive peptide aseppvldvkrpflc and its preparation method and application

A technology of bioactive peptides and sequences, applied in the field of protein, to achieve the effect of improving function, improving ability and improving quality

Active Publication Date: 2022-03-29
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no research on the function of Cytochrome b-c1 complex subunitRieske, mitochondrial protein polypeptide fragments in the prior art

Method used

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  • A kind of bioactive peptide aseppvldvkrpflc and its preparation method and application
  • A kind of bioactive peptide aseppvldvkrpflc and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 The artificial synthesis of active peptide ASEPPVLDVKRPFLC

[0033] 1. Synthesis of Bioactive Peptides

[0034] Synthetic bioactive peptide ASEPPVLDVKRPFLC.

[0035] 2. Confirmation of Bioactive Peptides

[0036] 1) UPLC analysis

[0037] The UPLC conditions are as follows:

[0038] Instruments: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0039] Column specification: BEH C18 column

[0040] Flow rate: 0.4mL / min

[0041] Temperature: 50°C

[0042] UV detection wavelength: 210nm

[0043] Injection volume: 2μL

[0044] Gradient conditions: liquid A: water containing 0.1% formic acid (v / v), liquid B: acetonitrile containing 0.1% formic acid (v / v)

[0045] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0046] 2) Mass spectrometry analysis

[0047] The mass spe...

Embodiment 2

[0061] Example 2 Immune Activity Experiment of Bioactive Peptides

[0062] 1. The experiment of bioactive peptide ASEPPVLDVKRPFLC promoting the secretion of cytokines by macrophages (ELISA method)

[0063] 1. Experimental reagents and instruments:

[0064] Reagents: experimental animals balb / c mice (male 6, 8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO company; cattle Serum albumin (bovine serum albumin, BSA), Genebase Company; mouse spleen lymphocyte-derived bioactive peptide ASEPPVLDVKRPFLC obtained in Example 1; ELISA cytokine rapid kit (TNF-α and IL-1β), Wuhan Boster Bioengineering Ltd.

[0065] Equipment: LRH, 250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL, 22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus; DragonWellscan MK3 microplate reader Labsystems.

[0066] 2...

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Abstract

The present invention relates to the field of proteins, in particular to a bioactive peptide ASEPPVLDVKRPFLC and its preparation method and application. The amino acid sequence of the bioactive peptide ASEPPVLDVKRPFLC is Ala‑Ser‑Glu‑Pro‑Pro‑Val‑Leu‑Asp‑Val‑Lys‑Arg ‑Pro‑Phe‑Leu‑Cys. The in vitro immune function regulation experiment verified that the biologically active peptide ASEPPVLDVKRPFLC has a good immune regulation function. The biologically active peptide ASEPPVLDVKRPFLC of the present invention can effectively inhibit the inflammation caused by oxidation in the body, promote the secretion of cytokines by macrophages, improve its function in the resting state of normal macrophages, improve the body's ability to resist external pathogen infection, reduce It is of great significance to develop food, health care products and medicines with immune regulation function to reduce the incidence of the body and improve the quality of life.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide ASEPPVLDVKRPFLC and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that, compa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00A61K8/66A61K38/43A61Q19/00A61P37/02A61P29/00A23L33/18
CPCC12N9/00A61K8/66A61P29/00A61P37/02A61Q19/00A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 张少辉占文静陈承余肖珊珊石毅洪志骏王娟李锡安梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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