Preparation and application of African horned melon extract for preventing and treating root-knot nematode incognita
A technology for Rhizoma incognita and African horned melon, which is applied in the preparation of an extract preparation of African horned melon for preventing and controlling Rhizoctonia incognita and the field of preparation thereof, and achieves good killing effect, wide practical value, and no destructive effect.
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Embodiment 1
[0036] Example 1: Preparation of African Horned Melon Extract Preparation for Controlling Meloidogyne incognita Used to prepare African Horned Melon Extract Preparation for Controlling Meloidogyne incognita, the preparation includes the extraction of stems, leaves and roots of African Horned Melon isolate.
[0037] Preferably, the African horned melon extract preparation for preventing and controlling root-knot nematode comprises 0.5-2 mg / mL extracts of stems, leaves and roots of African horned melon.
[0038] Further, the present invention also provides a method for preparing the above-mentioned ethanol extract of African horned melon stems, leaves and roots in the African horned melon extract preparation for preventing and controlling root-knot nematode, specifically prepared by the following steps:
[0039] (1) the stem, leaf and root of the fresh African horned melon for testing are removed and rolled into small sections with guillotine respectively;
[0040] (2) The samp...
Embodiment 2
[0046] Example 2: Determination of nematicidal activity of the African horned melon extract preparation used to prevent and control root-knot nematode incognita
[0047] (1) Determination of nematicidal activity
[0048] In a 96-well plate, add nematode suspension (about 200-300 nematodes) to each well, 65 μL of sterile water, 5 μL of sample solution, and the final concentration of the sample is 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL, 2 mg / mL , 4mg / mL, the blank control is to replace the sample solution with the same amount of sterile water, the positive control is to replace the sample solution with the same amount of 1mg / mL abamectin aqueous solution, repeat 3 times, add 200μL to the surrounding wells of the 96-well plate Moisturize with sterile water. After mixing, incubate at room temperature for 24 hours, count the number of dead nematodes under a fluorescent digital biological microscope, and the number of nematodes counted is not less than 100. The mortality and corrected morta...
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