Visual probe quality control method and system
A quality control method and probe technology, applied in the field of high-throughput sequencing, can solve the problem that the probe cannot be captured deeply into a gene, a fragment and a site.
Pending Publication Date: 2021-04-06
浙江绍兴鼎晶生物医药科技股份有限公司
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Problems solved by technology
[0003] At present, the research on probes in the industry is mainly focused on how to design probes based on the literature, but there is a lack of quality control and visualization of the designed probes, let alone the capture of probes to a certain gene, on a certain fragment and site
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[0128] In order to facilitate the understanding of the present invention, the present invention is illustrated by the following examples, but it does not constitute a limitation to the present invention.
[0129] The sample type in the present invention includes but not limited to any one or any combination of tissues, body fluids, blood or cells. The tissues include but not limited to paraffin tissue sections, paraffin tissue rolls, paraffin-embedded tissues, fresh tissues preserved in preservative solution, fresh tissues preserved in formalin, and fresh tissues preserved in alcohol. Body fluids include but not limited to cerebrospinal fluid and pericardial effusion. Blood includes but not limited to whole blood and cfDNA blood, and cells include but not limited to oral mucosal epithelial cells and peritoneal water cells.
[0130] Such as figure 1As shown, the visualized probe quality control system of the present invention includes a main control module and four sub-modules...
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The invention belongs to the technical field of high-throughput sequencing, and particularly relates to a visual probe quality control method and system. The method comprises the steps: building a visual data set and a significance data set of a historical sample, carrying out the integration and comparison of the sequencing information of a new sample and the historical sample, and carrying out the probe capture quality evaluation and visualization of the new sample. The system comprises a main control module, a data receiving module, a data integration module, a data visualization module and a historical data updating module, wherein the main control module is responsible for information transmission among the data receiving module, the data integration module, the data visualization module and the data updating module, and all the modules are called. Through the method and the system provided by the invention, convenience is provided for a user to quickly and efficiently understand probe capture conditions under different dimensions, a new reference basis is provided for probe design and improvement and selection and improvement of experimental processes and conditions, and meanwhile, a new reference evidence is provided for quality evaluation of high-throughput sequencing; and the credibility of a high-throughput sequencing report is improved.
Description
technical field [0001] The invention belongs to the technical field of high-throughput sequencing, and in particular relates to a visualized probe quality control method and system. Background technique [0002] As the number of cancer patients increases year by year, high-throughput sequencing technology, as one of the means of cancer detection, is gradually becoming popular. The accuracy of sequencing has a crucial impact on the identification of mutated genes and the provision of subsequent treatment options. During the sequencing process, it is necessary to use probes to capture base sequences, and the capture efficiency of probes directly affects the accuracy of high-throughput sequencing. How to enable researchers to understand the capture status of probes conveniently and quickly, identify, screen and check probes with low capture efficiency or abnormality, and improve probes based on probe capture status have become one of the key steps in the current high-throughpu...
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IPC IPC(8): G16B30/00G16B45/00G16B40/00
CPCG16B30/00G16B40/00G16B45/00
Inventor 丁首太史善甫何乐思严志祥沈伟强罗鹏蔡丽君
Owner 浙江绍兴鼎晶生物医药科技股份有限公司