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A method for efficient recovery of protein from excess sludge

A technology for excess sludge and protein, which is applied in peptide preparation methods, plastic recycling, sludge treatment, etc., can solve the problems of inapplicable protein recovery, complex protein components, and low protein content, so as to promote sludge dispersion and avoid Effects of excessive consumption, rapid biodegradability

Active Publication Date: 2022-08-09
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with distiller's grain waste, potato processing wastewater, and seafood processing wastewater, the protein content is low and the protein composition is complex
In addition, the composition of WAS is complex, and various influencing substances such as heavy metals and antibiotics will be produced during the hydrolysis process.
Therefore, existing protein recovery methods are not suitable for the recovery of proteins in WAS

Method used

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  • A method for efficient recovery of protein from excess sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]The present embodiment is a method for efficiently recovering protein from excess sludge (WAS), the method comprising the following steps:

[0033] Step 1: After the sludge taken out from the secondary sedimentation tank of the sewage treatment plant is allowed to stand at 4°C for 24 hours, the supernatant is discharged with a straw, and the sludge is filtered with a sieve with an aperture of 1 mm. The impurities in the sludge were screened out, and finally the treated sludge was stored at 4°C for subsequent experiments, and the SS of WAS was adjusted to be about 10,000 mg / L to obtain excess sludge. This treatment process is mainly to screen out the impurities in the WAS and prepare for the subsequent pretreatment.

[0034] Step 2: Take the treated excess sludge WAS in an anaerobic reactor with an effective volume of 500mL, adjust the pH of WAS to 10.0±0.2, and then add 0.3g / g SS APG at a temperature of 35±1℃ After the reaction was carried out, the reaction bottle was f...

Embodiment 2

[0040] The steps in this example are the same as those in Example 1, except that the dosage of APG in this example is 0.1, 0.2, 0.4, and 0.5 g / gSS, and the recovery rate of protein in this example is less than 90%. %, but above 80%.

[0041] Under the conditions of APG dosage of 0.3g / gSS and pH of 10.0, the hydrolysis effect of WAS was the best, the released protein content was high, and the sludge disintegration effect was obviously improved. Compared with the unpretreated sludge, when the dosage of APG was 0.3g / gSS and the pH was 10.0±0.2, the dissolution amount of SCOD of WAS increased from 200.2mg / L to 4500.0mg / L, and the dissolution amount of protein From 17.1mg / L to 1009.7mg / L, the dissolved amount of polysaccharide increased from 10.7mg / L to 663.9mg / L, the SCOD content increased by 22.5 times, the protein content increased by 59.0 times, and the polysaccharide content increased by 61.87 times.

Embodiment 3

[0043] The steps in this embodiment are the same as those in Embodiment 1, except that the preprocessing time in this embodiment is 0, 4, 8, 12, 24, 32, and 48 hours. During WAS hydrolysis, the highest concentrations of SCOD, protein and polysaccharide increased with the increase of APG dosage, but changed little with time. When the reaction time was 2h, the highest concentrations of SCOD, protein and polysaccharide were 3250.1, 798.9, and 478.1 mg / L, while the highest concentrations of SCOD, protein and polysaccharide in the blank group were only 200.2, 17.2, and 10.9 mg / L. When the reaction time is higher than 8h, the dissolution amount of protein decreases, which is not conducive to the efficient recovery of protein.

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Abstract

The present invention is a method for efficiently recovering protein from excess sludge. The method comprises the following steps: adding excess sludge WAS into a reaction flask, adding APG in an amount of 0.1-0.5g / gSS, adjusting the initial The pH value is 4.0 to 11.0; after the reaction flask is driven by oxygen and nitrogen for 2-5min, the reaction flask is sealed, and the WAS is hydrolyzed by constant temperature shaking culture; after the pretreated WAS is centrifuged, the excess sludge WAS is completely precipitated , after taking the supernatant, then adding carrageenan to the supernatant, the mass ratio of protein and carrageenan obtained by WAS hydrolysis is 25-1:1, and the pH is adjusted to 1.0-3.0, and the temperature is 30-37 After shaking at ℃ for 15 min, the carrageenan, protein and acid were fully mixed; the mixed solution was centrifuged, and the lower precipitate was the recovered protein. The technology of the invention adopts the technology of combining the complex coagulation method and the acid-base precipitation method after hydrolysis, so that the protein can be recovered to the greatest extent through the combined action of the two.

Description

technical field [0001] The invention belongs to the technical field of solid waste treatment, and in particular relates to a method for efficiently recovering proteins from excess sludge. Background technique [0002] It is estimated that by 2020, the sludge output in my country will reach 80 million tons, and the disposal of waste activated sludge (WAS) has attracted widespread attention. The conventional treatment method not only causes waste of resources, but also increases the burden on the environment. Therefore, methods for efficiently recovering resources in sludge and improving sludge treatment efficiency have broad application prospects. Sludge contains a lot of recyclable resources, such as protein, heavy metals, etc. In addition, the protein and cations in the sludge also have a great influence on the surface properties, sedimentation and dewatering performance of the sludge. Therefore, the recovery and reuse of protein in WAS can not only meet the reduction ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/32C07K1/14C02F11/127C02F11/02C02F11/00
CPCC07K1/32C07K1/145C02F11/02C02F11/00C02F11/127Y02W30/62
Inventor 李相昆谢红伟刘改革杨瑛俊刘富荣孙钰洁李佳泽
Owner HEBEI UNIV OF TECH
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