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Tomato root-knot nematode and tobacco mosaic virus resistance gene markers and methods

A tomato root-knot nematode and disease-resistant gene technology, applied in biochemical equipment and methods, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problems of insufficient judgment accuracy and misjudgment, and achieve high accuracy The effect of high, strong specificity, and strong commercial application value

Active Publication Date: 2022-05-20
江苏绿港现代农业发展股份有限公司
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Problems solved by technology

[0004] But the existing labeled primers SW-5-2-F / SW-5-2-R, the accuracy of its judgment is not enough, there are some cases of misjudgment

Method used

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  • Tomato root-knot nematode and tobacco mosaic virus resistance gene markers and methods
  • Tomato root-knot nematode and tobacco mosaic virus resistance gene markers and methods
  • Tomato root-knot nematode and tobacco mosaic virus resistance gene markers and methods

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Embodiment 1

[0050] Using tomato backbone parents independently selected by Jiangsu Lvgang Modern Agriculture Development Co., Ltd. Mark the reported primers SCAR-1-F / SCAR-1-R and SW-5-2-F / SW-5-2-R for PCR amplification, agarose gel electrophoresis, and the agarose gel electrophoresis The PCR products were cloned and sequenced, and two pairs of high-efficiency primers InMi1-F / InMi1-R and InMi2-F / InMi2-F of the tomato root-knot nematode resistance gene (Mi-1.2) were designed using the software Primer 5; Three pairs of high-efficiency primers InSW1-F / InSW1-R, InSW2-F / InSW2-R and InSW3-F / InSW3-R for mosaic virus resistance gene (SW-5b). The purpose of the present invention is to design more specific primers according to the conserved sequence of the reported disease-resistant gene, and detect tomato root-knot nematode disease-resistant gene (Mi-1.2) and tomato tobacco mosaic virus disease-resistant gene (SW -5b), and the primer fragment is shorter, the amplification efficiency is higher, and...

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Abstract

The invention relates to a gene marker and method for resistance of tomato root-knot nematode disease and tobacco mosaic virus disease, and two pairs of high-efficiency primers InMi1-F / InMi1 of tomato root-knot nematode disease resistance gene (Mi-1.2) are proposed. ‑R and InMi2‑F / InMi2‑R, 3 pairs of high-efficiency primers InSW1‑F / InSW1‑R, InSW2‑F / InSW2‑R and InSW3‑F for tomato tobacco mosaic virus disease resistance gene (SW‑5b) / InSW3‑R, which can more accurately judge the resistance and susceptibility of plants. The primer and method for the tomato disease resistance gene related marker of the invention can detect the PCR product by polyacrylamide gel electrophoresis, the amplification band pattern is clear, and there are multiple primers that can be repeated, and the accuracy is higher.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to a gene marker and method for resistance to tomato root-knot nematode and tobacco mosaic virus disease. Background technique [0002] Tomato (Lycopersicon esculentum Mill.) is an important vegetable crop integrating fresh food, vegetable use and ornamental. It has the advantages of high nutritional value, wide adaptability, easy cultivation and high economic benefits. It is widely planted all over the world. my country is one of the largest tomato production and consumption countries in the world. In recent years, with the rapid development of the tomato industry, continuous innovation and enrichment of cultivation methods, and comprehensive reform of cultivation techniques, tomato production has become an important way for vegetable farmers to increase their income and get rich and earn foreign exchange through export. The tomato market has huge potential, and the econo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 刘梦华阿门卢霞杨雪张文婷司龙亭胡芳辉
Owner 江苏绿港现代农业发展股份有限公司
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