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A kind of starworm ace inhibitory peptide and its preparation method and application

A technology of gramella and inhibitory peptides, which is applied in the field of gramella ACE inhibitory peptides and its preparation, can solve the problems of high production costs, achieve good antihypertensive effect, good enzymatic hydrolysis effect, and simple preparation process

Active Publication Date: 2022-04-22
MARINE MEDICAL RES INST OF GUANGDONG ZHANJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing antihypertensive drugs are artificially synthesized chemical drugs, which are prone to produce various side effects during clinical application, and the production cost is relatively high

Method used

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  • A kind of starworm ace inhibitory peptide and its preparation method and application
  • A kind of starworm ace inhibitory peptide and its preparation method and application
  • A kind of starworm ace inhibitory peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation and purification of ACE-inhibitory peptides from A. graminis

[0040](1) Turn the fresh starworm over to remove the viscera, wash it, weigh 500g, grind it with a chopping machine; add 5L of pure water, mix well, and adjust the pH to 7.5-9.5 with 1% sodium hydroxide solution; appropriate amount Enzyme powder (trypsin-neutral protease = 3:1, w / w) is dissolved in pure water to form a slurry to make enzyme powder slurry; slowly add the enzyme according to 0.5%-1% of the weight of the gridworm In the insect liquid, stir and mix evenly while adding, set the temperature at 45°C, and enzymatically hydrolyze for 4-6 hours;

[0041] On the basis that the pH is 7.5, the amount of enzyme added is 1% of the mass of the starworm, and the enzymatic hydrolysis time is 4 hours, the ACE inhibition rate of the enzymatic hydrolyzed product is used as an index to carry out a single factor experiment. One parameter is changed each time, and the pH is studied. 7.5, 8.5, 9.5, t...

Embodiment 2

[0056] Preparation of Buccal Tablets of Fangge Xing Chong Antihypertensive Peptide

[0057] 1 recipe

[0058] Main ingredient: 10-30% of the square starworm antihypertensive peptide

[0059] Excipients: one or more of lactose powder, maltodextrin, microcrystalline cellulose, 25-45%

[0060] Sweetener: one or more of maltitol, sorbitol, sucrose, xylitol, 15-28%

[0061] Sour agent: one or more of citric acid, malic acid, lactic acid, 5-10%

[0062] 2 method

[0063] The main material, auxiliary material, sweetener and sour agent are mixed, 50%-70% ethanol is slowly sprayed on the mixture as a wetting agent, and at the same time, it is constantly stirred evenly to make a soft material with moderate hardness. Use an 18-mesh sieve as a tool for making granules, and press through an 18-mesh sieve to make the soft material into granules to prepare the soft material. Soft materials are required to form a ball when held, and to disperse when touched. After the soft material is p...

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PUM

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Abstract

The invention discloses an ACE-inhibiting peptide of Acarina platica and its preparation method and application, belonging to the field of biotechnology. The present invention, through single factor experiments and orthogonal experiments, has determined that the optimal process parameters for enzymatic hydrolysis of the gridworm are compound enzyme powder (trypsin-neutral protease=3:1), solid-liquid ratio of 1:10, enzyme The mass ratio to the substrate is 1%, the enzymolysis pH is 9.5, the enzymolysis temperature is 45°C, and the enzymolysis time is 4h. Peptide amino acid sequence, using the obtained ACE-inhibiting peptide to prepare the buccal tablet of Pterostella chinensis antihypertensive peptide. The invention provides a new antihypertensive peptide of the phalaenopsis, determines the best preparation process, and lays the foundation for further preparation of drugs with antihypertensive effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gramina ACE inhibitory peptide and its preparation method and application. Background technique [0002] With the development of my country's economy and the continuous improvement of people's living standards, the number of patients with hypertension in our country is also increasing. It is estimated that the number of hypertensive patients in my country may exceed 300 million in 2020. Most of the existing antihypertensive drugs are artificially synthesized chemical drugs, which are prone to produce various side effects in the clinical application process, and the production cost is relatively high. Therefore, low cost, high safety, and good antihypertensive effect of biological antihypertensive peptides have become the focus of research in recent years. [0003] Due to the particularity of the marine ecological environment (such as high pressure, low temperature, etc.), marine ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/22A61K38/08A61P9/12A23L33/18A23P10/28
CPCC07K7/06A61P9/12A23L33/18A23P10/28A61K38/00A23V2002/00A23V2250/55A23V2200/326
Inventor 戚怡吕应年罗辉林芊杏唐晓宁刘欢
Owner MARINE MEDICAL RES INST OF GUANGDONG ZHANJIANG
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