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Method for evaluating micro-plastic pollution level based on physiological indexes of drosophila melanogaster

A technology of pollution levels and microplastics, applied in data processing applications, instruments, resources, etc., can solve problems such as long experiment cycles

Pending Publication Date: 2021-04-13
HANGZHOU DIANZI UNIV
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  • Description
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Problems solved by technology

The evaluation of the pollution level of microplastics by model organisms can be judged by the lethality rate, but the experiment period is longer

Method used

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  • Method for evaluating micro-plastic pollution level based on physiological indexes of drosophila melanogaster
  • Method for evaluating micro-plastic pollution level based on physiological indexes of drosophila melanogaster
  • Method for evaluating micro-plastic pollution level based on physiological indexes of drosophila melanogaster

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Embodiment Construction

[0027] The technical solutions of the present invention will be further explained below through specific examples.

[0028] The method for assessing the level of microplastic pollution based on the physiological indicators of Drosophila according to the embodiment of the present invention includes the following steps:

[0029] Step 1: first collect fruit flies at the same developmental stage, and select healthy fruit flies for experiments;

[0030] Step 2: Production of microplastic-contaminated medium;

[0031] Step 3: Place healthy fruit flies in a microplastic-contaminated culture medium and culture them continuously for 5 days in a constant temperature incubator with a humidity of 60% and a temperature of 25 degrees, collect the number of eggs laid by fruit flies, and measure microplastics at different concentrations. Reproductive toxicity caused by plastic pollution;

[0032] Step 4: Place the healthy fruit flies in the microplastic-exposed medium, culture them continuo...

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Abstract

The invention belongs to the technical field of micro-plastic pollution assessment, and particularly relates to a method for assessing micro-plastic pollution level based on physiological indexes of drosophila melanogaster, which comprises the following steps: (1) collecting drosophila melanogaster in the same development stage, and selecting healthy drosophila melanogaster; (2) preparing a micro-plastic polluted culture medium; (3) putting healthy drosophila melanogaster into a micro-plastic pollution culture medium, continuously culturing for 5 days at a constant temperature, collecting the egg laying quantity of the drosophila melanogaster, and determining the reproductive toxicity caused by micro-plastic pollution under different concentrations; (4) putting healthy drosophila melanogaster into a micro-plastic polluted culture medium, continuously culturing for 20 days at a constant temperature, and measuring the behavior activity, the food intake and the in-vivo metabolic substance content of the drosophila melanogaster; and (5) performing activity correlation analysis on reproductive toxicity, behavioral activity, food intake and in-vivo metabolic substance content by using a Ttest. According to the invention, scientific theoretical support is provided for evaluating the safety problem of micro-plastic pollutants on an ecological system and human health.

Description

technical field [0001] The invention belongs to the technical field of microplastic pollution assessment, and in particular relates to a method for assessing microplastic pollution levels based on physiological indicators of fruit flies. Background technique [0002] Environmental pollution has become a common problem all over the world. In recent years, microplastics have received global attention as a new type of environmental problem. At the second United Nations Environment Assembly held in 2015, microplastic pollution was listed as the second largest scientific issue in the field of environmental and ecological science research, and became a major global environmental problem alongside global climate change and ozone depletion. Pollution is getting worse. [0003] Microplastics not only exist in the environment, but can also enter the human body through food delivery. Studies have shown that 11.6 billion microplastics and 3.1 billion nano-microplastics will be releas...

Claims

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Application Information

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IPC IPC(8): G06Q10/06G06Q50/26A01K67/033
CPCG06Q10/06393G06Q10/0635G06Q50/26A01K67/033
Inventor 梁博颖沈洁张大可钟立超唐昊
Owner HANGZHOU DIANZI UNIV
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