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InDel marker fingerprint of shiitake Minfeng No.1 strain and construction method thereof

A technology of fingerprint spectrum and construction method, which is applied in the field of detection of Lentinus edodes strains, can solve the problems of the same name of different species, damage to the intellectual property rights of mushroom breeders, and the same name of the same species.

Inactive Publication Date: 2021-04-23
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country's shiitake mushroom cultivation scale has been further expanded, but with the rapid development of the shiitake mushroom industry in my country, the problem of "same species with different names, different species with the same name" has become increasingly prominent, which not only damages the intellectual property rights of shiitake mushroom breeders, but also causes damage to producers. It has created great risks and created hidden dangers for the further development of the shiitake mushroom industry.

Method used

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  • InDel marker fingerprint of shiitake Minfeng No.1 strain and construction method thereof
  • InDel marker fingerprint of shiitake Minfeng No.1 strain and construction method thereof
  • InDel marker fingerprint of shiitake Minfeng No.1 strain and construction method thereof

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Experimental program
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Embodiment 1

[0064] (1) Mycelia culture: transfer the mushroom mycelia to the potato glucose medium PDA, cultivate in the dark at 23-22°C, and collect the mycelium after 10 days;

[0065] (2) Extraction of genomic DNA: extract the genomic DNA of the above-mentioned hyphae with the CTAB method, detect the concentration and purity of the total genomic DNA by ultraviolet spectrophotometry, and adjust the concentration of the sample DNA to be 20 to 30 ng / uL;

[0066] The CTAB method for extracting the genomic DNA from mycelium includes:

[0067] ①Take the freeze-dried shiitake mycelia in a 2mL centrifuge tube, and put 2-3 steel balls into it;

[0068] ②Put the centrifuge tube into a multi-sample tissue grinder, set the frequency to 60Hz, and grind for 30s to a uniform powder;

[0069] ③ Add 2×CTAB extract preheated at 62°C for 1 hour, keep warm at 62°C for 60 minutes, and shake gently once every 20 minutes;

[0070] ④ Centrifuge at 12000rpm, 4°C for 20min, dispense the supernatant into two t...

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Abstract

The invention relates to an InDel marker fingerprint of a shiitake Minfeng No.1 strain and a construction method thereof. The fingerprint is composed of seven pairs of InDel markers developed after whole-genome sequencing of the shiitake. The InDel marker fingerprint of the shiitake Minfeng No.1 strain can be used for specific identification of the shiitake Minfeng No.1 strain, has the advantages of short detection time, high accuracy and good repeatability, and has the specificity of the shiitake Minfeng No.1 strain among 44 collected shiitake strains.

Description

technical field [0001] The invention belongs to the detection field of shiitake mushroom strains, and in particular relates to an InDel-marked fingerprint of shiitake mushroom Minfeng No. 1 strain and a construction method thereof. Background technique [0002] Mushrooms (Lentinula edodes (Berk.) Pegler) are popular among consumers because of their high nutritional value and medicinal value. China is the first country in the world to carry out the artificial cultivation of shiitake mushrooms, with a cultivation history of more than 800 years. At present, shiitake mushrooms are the most widely cultivated and the highest yield mushroom species in China. According to statistics from the China Edible Fungi Association, the total output of edible fungi in China in 2018 was 38.4204 million tons, of which the total output of shiitake mushrooms was 10.4312 million tons, accounting for 27.15% of the total domestic output of edible fungi and more than 90% of the world's total output o...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12Q1/04C12N15/11
Inventor 章炉军于海龙沈秀芬尚晓冬宋春艳张美彦谭琦周峰姜宁董浩然
Owner SHANGHAI ACAD OF AGRI SCI