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A sucrose biosensor chip with specific isoforms

A biosensing and sucrose technology, which is applied in the field of preparation of sucrose biosensing chips, can solve the problems of unfavorable daily detection, cumbersome steps, and long detection cycle, and achieve the effects of accelerating electron transmission rate, avoiding enzyme pollution, and good performance

Active Publication Date: 2022-03-25
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive intake of sucrose can cause disorders of body functions and lead to diseases, so the detection of sucrose concentration is very important
[0003] Nowadays, the only way to detect sucrose is to hydrolyze sucrose into glucose and fructose by acid hydrolysis, and then detect the concentration of glucose by titration or chromatography to infer the concentration of sucrose. The traditional method steps are too cumbersome and the detection accuracy is not high. Chromatography The cost of the method is high and the detection cycle is long, which is not conducive to daily detection
At the same time, a large amount of chemical reagents need to be used in the detection process, which will cause environmental pollution

Method used

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  • A sucrose biosensor chip with specific isoforms
  • A sucrose biosensor chip with specific isoforms

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for preparing a sucrose biosensing chip with a specific configuration, comprising the following preparation steps:

[0029] (1) Configure 10mM CuSO 4 Solution and the melamine solution of 5mM are mixed in equal volumes, and the mixed solution is centrifuged with a centrifuge, the centrifugation rate is 6000r / min, and the centrifugation time is 30min to obtain the copper melamine complex nano-slurry;

[0030] (2) the copper melamine complex nano-slurry of synthesis is mixed with carbon slurry by mass ratio 1:1, and make it mix evenly, prepare copper melamine complex-carbon mixed slurry;

[0031] (3) if figure 2 As shown, the base electrode is printed on the bottom plate material by screen printing technology, and the working electrode area of ​​area 1 has an area of ​​0.15cm 2 , the slurry used for printing is the copper melamine complex-carbon mixed slurry prepared in step 2); the area 2 reference electrode area has an area of ​​0.05cm 2 , the paste used fo...

Embodiment 2

[0036] A method for preparing a sucrose biosensing chip with a specific configuration, comprising the following preparation steps:

[0037] (1) Configure 20mM CuSO 4 Solution and the melamine solution of 40mM are mixed in equal volumes, and the mixed solution is centrifuged with a centrifuge, the centrifugation rate is 8000r / min, and the centrifugation time is 15min to obtain the copper melamine complex nano-slurry;

[0038] (2) the copper melamine complex nano-slurry of synthesis is mixed with carbon slurry by mass ratio 1:9, and make it mix evenly, prepare copper melamine complex-carbon mixed slurry;

[0039] (3) if figure 2 As shown, the base electrode is printed on the bottom plate material by screen printing technology, and the working electrode area of ​​area 1 has an area of ​​0.15cm 2, the slurry used for printing is the copper melamine complex-carbon mixed slurry prepared in step 2); the area 2 reference electrode area has an area of ​​0.05cm 2 , the paste used fo...

Embodiment 3

[0044] A method for preparing a sucrose biosensing chip with a specific configuration, comprising the following preparation steps:

[0045] (1) Configure 10mM CuSO 4 Solution and the melamine solution of 50mM are mixed in equal volumes, and the mixed solution is centrifuged with a centrifuge, the centrifugation rate is 12000r / min, and the centrifugation time is 5min to obtain the copper melamine complex nano-slurry;

[0046] (2) the copper melamine complex nano-slurry of synthesis is mixed with carbon slurry by mass ratio 1:49, and make it mix evenly, prepare copper melamine complex-carbon mixed slurry;

[0047] (3) if figure 2 As shown, the base electrode is printed on the bottom plate material by screen printing technology, and the working electrode area of ​​area 1 has an area of ​​0.15cm 2 , the slurry used for printing is the copper melamine complex-carbon mixed slurry prepared in step 2); the area 2 reference electrode area has an area of ​​0.05cm 2 , the paste used ...

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Abstract

The invention provides a sucrose biosensing chip with a specific configuration, which belongs to the technical field of sucrose biomonitoring. Mix the synthesized copper melamine complex nano slurry with carbon slurry to prepare copper melamine complex-carbon mixed slurry for printing to speed up the electron transfer rate and adjust the amount of enzyme loaded on the chip to control the detection sensitivity and detection linear range. The screen printing technology is adopted, and the preparation method is simple and convenient to operate and can be mass-produced with low cost. The prepared sucrose biosensing chip has good performance, and has good reproducibility, adaptability and stability. The specific working electrode area can separate the modified enzymes to avoid enzyme contamination. Compared with the cumbersome steps of the traditional detection method of sucrose and the environmental pollution of the traditional detection method, it has the advantages of high efficiency, safety, simplicity and economy, and is of great significance to human health, snack manufacturing, fermentation industry and pharmaceutical industry.

Description

technical field [0001] The invention relates to a preparation method of a sucrose biosensing chip with a specific configuration, belonging to the technical field of sucrose biomonitoring. Background technique [0002] Sucrose is the main product of photosynthesis and is widely distributed in plants, especially in beet, sugarcane and fruit. Sucrose is the main form of sugar storage, accumulation and transport in plants. The white sugar and brown sugar that are usually eaten are all sucrose. Sucrose is formed by the dehydration condensation of one molecule of glucose and one molecule of fructose. It is easily soluble in water and difficult to dissolve in ethanol. Its sweetness is second only to fructose. After the human body ingests sucrose, it is converted into glucose and fructose by the body, and a part of the glucose is transported to the whole body along with the blood circulation, where it generates energy and supplies brain tissue functions and muscle activities of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/02
CPCG01N27/021
Inventor 储震宇赵强金万勤
Owner NANJING TECH UNIV
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