Complex microbial inoculant capable of reducing nitrous oxide emission and application of complex microbial inoculant

A compound bacterial agent, nitrous oxide technology, applied in the application, bacteria, preparation of organic fertilizers and other directions, can solve the problems of difficult promotion, secondary pollution, slow decomposition rate of organic matter, etc., to reduce environmental pollution and protect the ecological environment. , the effect of improving the quality of compost

Active Publication Date: 2021-04-30
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the adsorption capacity of physical adsorption method is quite different, and the adsorption effect on nitrous oxide is general and unstable.
The chemical additive method can reduce a part of ammonia volatilization, but the removal effect of nitrous oxide is not obvious, and it is easy to cause secondary pollution; changing the carbon-nitrogen ratio of compost has limited effect on reducing the emission of nitrous oxide, and the carbon-nitrogen ratio is too high. The growth of microorganisms slows down the decomposition rate of organic matter, which makes it difficult to promote it in production; the impact of adding single bacteria on indigenous microorganisms is relatively small, and the effect of reducing nitrous oxide emissions is poor and unstable; in addition, although the composting process parameters The adjustment of nitrous oxide, such as adjusting the pH value, ventilation time, turning frequency, film covering, etc., can also reduce the emission of nitrous oxide, but the diversity of composting technology and scale makes optimizing parameters not a way to reduce nitrous oxide emission effective way

Method used

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  • Complex microbial inoculant capable of reducing nitrous oxide emission and application of complex microbial inoculant

Examples

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Effect test

Embodiment 1

[0035] In this example, a composite bacterial agent was prepared, including Marivirga tractuosa, Prevotella denticola, and Desulfotomaculum ruminis. Marivirga tractuosa is Marivirga tractuosa DSM4126, Prevotella adenticola is Prevotella denticola F0289, Desulfotomaculum ruminis is rumen desulfurizing enteric bacteria DSM2154.

[0036] M. marinum DSM4126, Prevotella tooth-dwelling F0289, and Desulfurized enterobacterium rumen DSM2154 were taken, amplified by pure culture, and then made into freeze-dried bacteria powder with skimmed milk powder. Carry out the live bacteria count of freeze-dried powder again, guarantee that the content of three strains of bacteria is 10 8 ~10 9CFU / g. Mix according to the mass ratio of Marivirga tractuosa, Prevotelladenticola and Desulfotomaculum ruminis at a ratio of 2:1:1.

Embodiment 2

[0038] In this example, a composite bacterial agent was prepared, including Marivirga tractuosa, Prevotella denticola, and Desulfotomaculum ruminis. Marivirga tractuosa is Marivirga tractuosa DSM4126, Prevotella adenticola is Prevotella denticola F0289, Desulfotomaculum ruminis is rumen desulfurizing enteric bacteria DSM2154.

[0039] M. marinum DSM4126, Prevotella tooth-dwelling F0289, and Desulfurized enterobacterium rumen DSM2154 were taken, amplified by pure culture, and then made into freeze-dried bacteria powder with skimmed milk powder. Carry out the live bacteria count of freeze-dried powder again, guarantee that the content of three strains of bacteria is 10 8 ~10 9 CFU / g. Mix according to the mass ratio of Marivirga tractuosa, Prevotelladenticola and Desulfotomaculum ruminis at a ratio of 1:1:1.

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Abstract

The invention discloses a complex microbial inoculant capable of reducing nitrous oxide emission in a composting process and an application of the complex microbial inoculant. The complex microbial inoculant comprises marivirga tractuosa, prevotella denticola and desulfotomaculum ruminis. Through the synergistic effect of the three strains, greenhouse gas nitrous oxide can be reduced into harmless nitrogen more efficiently. The maximum emission reduction of oxygen and nitrogen oxide reaches 402 [mu]g/kg. By adding the microbial inoculant, the emission of nitrous oxide in the composting process is effectively controlled, and the ecological environment is protected.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a composite bacterial agent for reducing nitrous oxide emission and an application thereof. Background technique [0002] With the rapid development of industrialization and urbanization in our country, the amount of sewage treatment is increasing, and the amount of sludge generated in the process of sewage treatment is also increasing year by year. According to statistics, my country produces more than 50 million tons of urban sludge with a water content of 80% annually. These sludges contain a large amount of harmful substances such as refractory organic matter, parasite eggs, and pathogenic microorganisms. In order to prevent pollutants from migrating to agricultural land and groundwater Potential risk to human health, treatment and disposal of sludge is necessary. [0003] Composting is a cost-effective sludge stabilization biotechnology that both inactivat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F17/80C05F15/00C12R1/01
CPCC12N1/20C05F5/002C05F17/20C05F17/80C05F7/00Y02W30/40
Inventor 廖汉鹏白玉丹崔鹏周顺桂陈志
Owner FUJIAN AGRI & FORESTRY UNIV
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