Real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection primer for identifying FAdV-4 and DAdV-4 and kit thereof
A technology for real-time fluorescence quantification and detection of primers, applied in the field of animal epidemiology
Active Publication Date: 2021-04-30
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology
At present, there is no real-time fluorescent quantitative PCR detection method that can identify two types of adenoviruses (FAdV-4 and DAdV-4) of the Adenoviridae family Avian Adenovirus genus that infect poultry with only one set of primers at home and abroad. Primer research reports, the establishment of this invention can fill in the gaps in related fields at home and abroad
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Embodiment 1
[0042] 1. Related test pathogens
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The invention provides a real-time fluorescent quantitative PCR (polymerase chain reaction) detection primer for identifying fowl adenovirus type 4 (FAdV-4) and duck adenovirus type 4 (DAdV-4) and a kit thereof, and the sequence of the primer is shown as SEQ ID NO.1-2. A detection method established by utilizing the primer has very high specificity and sensitivity. At present, a primer research report of a real-time fluorescent quantitative PCR detection method capable of identifying two types of adenoviruses (FAdV-4 and DAdV-4) of adenovirus family aviadenoviruses infecting poultry by only one group of primers does not exist at home and abroad, and the establishment of the real-time fluorescent quantitative PCR detection method disclosed by the invention can fill up the blank of related fields at home and abroad.
Description
technical field [0001] The invention relates to a real-time fluorescent quantitative PCR detection primer and a kit for differentiating avian adenovirus type 4 (FAdV-4) and duck adenovirus type 4 (DAdV-4), belonging to the field of animal infectious diseases. Background technique [0002] Duck adenovirus 4 (DAdV-4) is a new duck adenovirus disease discovered in southern my country in 2019. Analysis of the virus genome revealed that its main gene-encoded protein had the same nucleotide and amino acid homology as the duck type 1 adenovirus, duck type 2 adenovirus and duck type 3 adenovirus-related genes found in duck populations earlier. less than 80%. Genetic evolution analysis showed that DAdV-4 belongs to the genus Aviadenovirus in the family Adenoviridae, but it is in an independent genetic evolutionary branch, so it was named duckadenovirus 4 (DAdV-4). In 2020, our research team also confirmed the presence of duck type 4 adenovirus (DAdV-4) infection in duck populations...
Claims
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Login to View More IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6851C12Q2563/107C12Q2531/113
Inventor 万春和黄瑜陈翠腾施少华陈珍傅光华程龙飞陈红梅傅秋玲刘荣昌
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI