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Object-carrying glassware, fluorescence microscopic imaging device and use method

A technology of object-carrying glass and microscopic imaging, which is applied in the direction of measuring devices, fluorescence/phosphorescence, instruments, etc., can solve the problems of low success rate, shortage, time-consuming and labor-intensive operation, etc.

Pending Publication Date: 2021-04-30
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the traditional fluorescence microscope imaging device in the prior art. In the cell fluorescence imaging experiment, when it is necessary to observe the same position before and after treatment, due to the lack of markers, the operation is time-consuming and laborious and has a low success rate. To solve the technical problems, thereby providing a glassware with markers, a fluorescence microscopic imaging device with the glassware, and a method for using the fluorescent microscopic imaging device

Method used

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  • Object-carrying glassware, fluorescence microscopic imaging device and use method
  • Object-carrying glassware, fluorescence microscopic imaging device and use method
  • Object-carrying glassware, fluorescence microscopic imaging device and use method

Examples

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Embodiment 1

[0059] see Figure 1 to Figure 6 , a kind of object-loading glassware provided by the present embodiment, comprising: a body 1 and at least one fluorescent marker 11, the body 1 has a relative object-loading surface 10 and a positioning surface (not marked), and the object-loading surface 10 is suitable for placing the object to be Observing the sample (not shown) and setting the fluorescent marker 11, the positioning surface is suitable for fixing and positioning the object-carrying glassware on the electronically controlled stage of the fluorescence microscopy imaging device. The body 1 of the object-carrying glassware of this embodiment is an existing conventional confocal dish (such as figure 1 and Figure 4 shown) or slides (such as figure 2 and Figure 5 shown). The excitation wavelength and emission wavelength of the fluorescent marker 11 are different from the excitation wavelength and emission wavelength of the fluorescent substance of the sample to be observed, ...

Embodiment 2

[0066] For the fluorescent microscopic imaging device provided in this embodiment, see Figure 1 to Figure 8 , including an excitation light module (not shown), an electronically controlled stage, the object-carrying glassware of Example 1, an image acquisition module and a computer. Wherein the excitation light module is used to generate the laser beam as the excitation light; the electronically controlled stage includes an operating table 5, an object stage 2 that can be controlled by the operation table 5 to move in a horizontal plane, and an operable stage installed on the object stage 2. Table 5 controls the rotating module 3 that rotates relative to the object stage 2, and the operating table 5 can display the plane displacement of the object stage 2 and the rotation angle of the rotating module 3; The module 3 moves, and is used to place the sample to be observed, and the laser beam is focused on the fluorescent marker 11 on the object-carrying glassware and the sample ...

Embodiment 3

[0071] A method for using a fluorescence microscope imaging device provided in this embodiment includes the following steps:

[0072] S1. Fix the sample to be observed on the rotating module 3 of the electronically controlled stage;

[0073] S2. Find the fluorescent marker 11 on the object-carrying glassware in the field of view of the corresponding magnification, and judge whether the fluorescent marker 11 in the field of view matches the standard cross on the display screen or the imaging software interface. Control the horizontal displacement and rotation angle of the stage so that the fluorescent marker coincides with the standard cross, and then zero-adjust the displacement and rotation angle of the electronically controlled stage displayed at this moment to calibrate the origin position;

[0074] S3. Adjust the electronically controlled stage and find the target observation area of ​​the sample to be observed in the field of view, and record the displacement and rotation...

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Abstract

The invention relates to the technical field of microscopic imaging, and discloses an object-carrying glassware, a fluorescence microscopic imaging device and a use method. The object-carrying glassware comprises: a body having an object-carrying surface for carrying a to-be-observed sample; and at least one fluorescence marker arranged on the object carrying surface, wherein the fluorescence excitation wavelength and the emission wavelength of the fluorescence marker are different from the excitation wavelength and the emission wavelength of the fluorescent substance in the to-be-observed sample. The fluorescent marker is arranged, so that a reference standard is provided when a sample is observed, and a previously observed region of interest can be conveniently and quickly found. Meanwhile, the excitation wavelength and the emission wavelength of the fluorescent marker are set to be different from the characteristics of the fluorescent substance in the sample, so that comparison is formed, and the influence on the accuracy of the experiment is avoided.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to an object-carrying glass vessel, a fluorescent microscopic imaging device and a use method. Background technique [0002] In the fluorescence imaging experiment of cell or tissue slices, it is necessary to place the sample on the fluorescence imaging device to observe the position of interest and use the imaging device to display the fluorescence image. In addition, the camera device can also be used to take pictures for the use of scientific research papers. Some fluorescence imaging experiments may require observation of the same location before and after sample treatment. [0003] However, in the existing traditional fluorescence imaging experiments, samples such as cell or tissue slices need to be placed on a transparent glass loading vessel for observation, and then the transparent glass loading vessel with the sample is fixed on the stage. Determine the appropr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6458
Inventor 彭晓彭政刘浩宋一纨于德良袁玉峰聂凯旋
Owner SHENZHEN UNIV
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