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Method for measuring blood coagulation time

A technology of coagulation time and coagulation time, which is applied in measuring devices, biological tests, instruments, etc., can solve the problems of unrecorded time and achieve the effect of improving storage stability

Pending Publication Date: 2021-04-30
SEKISUI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, neither of the two documents describes the content related to the control of the mean normal prothrombin time (hereinafter, sometimes referred to as MNPT)

Method used

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  • Method for measuring blood coagulation time
  • Method for measuring blood coagulation time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Confirmation of the influence of cobalt chloride coexistence on MNPT

[0046] (1-1) Reagents and inspection materials

[0047] (1-1-1) Thromboplastin reagent

[0048] Prepare a solution containing tissue thromboplastin extract prepared from rabbit brain by acetone extraction and 1 mmol / L calcium chloride as a thromboplastin reagent.

[0049] (1-1-2) Cobalt chloride-added thromboplastin reagent

[0050] 0.0, 0.5, 1.0, 2.0, 3.0, and 4.0 mmol / L of cobalt chloride were added to the thromboplastin reagent, respectively, to prepare cobalt chloride-added thromboplastin reagents at respective concentrations.

[0051] (1-1-3) Normal inspection materials

[0052] Two different lots of normal citrated pooled plasma (Sekisui Chemical Co., Ltd.) were used.

[0053] (1-2) Determination method of prothrombin time

[0054] After incubating 50 µL of the normal plasma at 37°C, 100 µL of the cobalt chloride-added thromboplastin reagent at each concentration was added ther...

Embodiment 2

[0060] Example 2. Effects of the coexistence of various additives on the MNPT of the thromboplastin reagent and the storage stability of the reagent confirm

[0061] (2-1) Reagents and inspection materials

[0062] (2-2) Determination method of prothrombin time

[0063](2-1) and (2-2) were carried out in the same manner as in Example 1 except that the added substance was the metal salt shown in Table 2.

[0064] (2-3) Storage stability test method

[0065] The prothrombin time of normal plasma was measured after the thromboplastin reagent added with each of the additives was stored at 37° C. for 28 days to confirm changes in reagent performance.

[0066] (2-4) Results

[0067] The solidification time when each additive substance (1.0 mmol / L) was added was shortened compared to the solidification time when no additive substance (0 mmol / L) was added ([B] in Table 2). In addition, at 37° C., the solidification time after storage for 28 days was equivalent to that of the c...

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Abstract

There is a demand for a method for controlling MNPT. According to the present invention, MNPT can be controlled and adjusted to a desired time by causing a specific metal ion to coexist with tissue thromboplastin. In addition, when the specific metal ion is caused to coexist with tissue thromboplastin, the stability of a thromboplastin reagent can be improved when the thromboplastin reagent is stored in a solution state.

Description

technical field [0001] The present invention relates to a method for controlling the average normal prothrombin time and improving the storage stability of a blood coagulation time measuring reagent containing tissue thromboplastin in blood coagulation examination. Background technique [0002] In the management of anticoagulant therapy, monitoring based on prothrombin time (hereinafter, sometimes referred to as PT) is important. PT is measured using a thromboplastin reagent containing tissue thromboplastin and calcium ions. Although various types of this reagent are commercially available, the performance of each company's reagent is different, and the stability of the reagent when stored in a solution state is also poor. [0003] Patent Document 1 discloses a method of including a nickel compound in a thromboplastin reagent to adjust the International Sensitivity Index (ISI) to 1.5 or less, and a composition using the method. [0004] Patent Document 2 discloses a reagen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N33/86
CPCG01N33/86G01N2333/4701
Inventor 大西健悟坂场义正西尾朋久
Owner SEKISUI MEDICAL CO LTD
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