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Method for breeding grouper

A grouper and fertilized egg technology, applied in the field of animal breeding, can solve the problem of inability to fundamentally effectively cut off the vertical transmission of fish nodavirus, and achieve the effects of avoiding vertical transmission, low deformity rate and high hatching rate

Active Publication Date: 2021-05-04
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Although it can speed up the detection of Nodavirus, it cannot fundamentally effectively cut off the vertical transmission of Nodavirus in fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Collection of adult grouper cells

[0034] At 17:00, in the No. 4 and No. 5 broodstock breeding ponds of the farm, a gonad-mature saddle grouper was selected, weighing about 85 kg, and a small amount of sex cells were obtained by squeezing the abdomen. Microscopic examination showed sperm, and it was determined to be a male fish . About 90 milliliters of semen were obtained by extrusion, and stored in plastic tubes for later use. Samples were taken to detect fish nodavirus RNA and red sea bream iridescent virus DNA.

[0035] At 20:30 on the same day, in No. 7 and No. 8 broodstock breeding ponds, 13 gonad-mature brown grouper broodstock were selected, weighing about 6 kg-7 kg, with a full abdomen, and squeezed the abdomen to obtain a small amount of sex cells. Microscopic examination confirmed that 10 are female fish. The ovum cells were obtained by extrusion method, and the ovum cells of each broodstock were independently placed in plastic basins and numbered. Th...

Embodiment 2

[0045] 2.1 Collection of grouper mature cells: at 17:20 in the afternoon, in the No. 2 and No. 3 broodstock breeding ponds of the farm, select 2 gonad-mature saddleband grouper, weighing about 85 kg and 90 kg respectively, with a full abdomen , Squeeze the abdomen to obtain a small amount of sex cells, microscopic examination is sperm, and it is determined to be a male fish. About 100 milliliters and 90 milliliters of semen were obtained by extrusion method, respectively, and placed in plastic basins. Samples were taken to detect fish nodavirus RNA.

[0046] In No. 5 and No. 6 broodstock breeding ponds, 18 gonadal mature brown grouper broodstock were selected, weighing about 6-8 kg, with a full abdomen, and squeezed the abdomen to obtain a small amount of sex cells. Microscopic examination confirmed that 14 of them were female fish. The ovum cells were obtained by extrusion method, and the ovum cells of each broodstock were independently placed in plastic basins and numbered....

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Abstract

The invention discloses a method for breeding groupers. The method comprises the following steps that seminal fluid of the groupers and egg cells of female groupers are respectively collected; the seminal fluid is mixed with the egg cells, and is slightly stirred to obtain fertilized eggs; the fertilized eggs are placed in sand-filtered seawater for standing, then the fertilized eggs are flushed with the sand-filtered seawater, and semen, excreta and secretions of the parent fish on the fertilized eggs are cleaned; the density of the fertilized eggs is 5-10 c.mL <-1 >, micro-inflation is carried out, a 5% povidone-iodine solution is added according to the dosage of 15 * 10 <-6 > L <-1 >, medicated bath is carried out for 10-15 min, and finally disinfected embryos are collected; and the disinfected embryos are placed in disinfected seawater with the salinity of 30 for incubation at the density of 0.01-0.03 / ml, and the micro-inflation and the incubation are carried out. After fertilized eggs infected with fish nodavirus is cleaned and disinfected before a cleavage 4 cell stage, the RNA detection of the fish nodavirus is negative; finally, through incubation operation, larva fish without the fish nodavirus can be obtained, the incubation rate of the larva fish is high, the malformation rate is low, and vertical infection of the fish nodavirus can be effectively avoided.

Description

technical field [0001] The invention relates to the field of animal breeding, in particular to a grouper breeding method. Background technique [0002] Piscine nodaviruses (Viral nervous necrosis, VNN) infects grouper fish such as the oblique-banded grouper, brown-spotted grouper, saddle-banded grouper, and pearl grouper, and The main viruses in various growth stages of marine fish such as European wolf bass and cobia. Studies have shown that its transmission routes are mainly vertical transmission of broodstock, horizontal transmission of bait organisms and living environment, and the mortality rate after infection is over 90%, resulting in mass death of larvae, juveniles, juveniles or adult fish, which seriously inhibits the cultivation of rare and precious fish in seawater sustainable development of the industry. [0003] In the production of grouper seedlings, due to the vertical transmission of Noda virus in broodstock fish, the outbreak of viral diseases in larvae, j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10C02F1/50C02F103/08C02F103/20
CPCA01K61/10C02F1/50C02F2303/04C02F2103/08C02F2103/20Y02A40/81
Inventor 施钢潘传豪王忠良吴勇曾珍
Owner GUANGDONG OCEAN UNIVERSITY
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