Unlock instant, AI-driven research and patent intelligence for your innovation.

Milk somatic cell separation process optimization process

A milk somatic cell and separation process technology, which is applied in the optimization process field of milk somatic cell separation process, can solve cumbersome problems and achieve the effects of saving time, reducing operations and reducing manual interference

Inactive Publication Date: 2021-05-04
SOUTH CHINA AGRI UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The milk somatic cell image segmentation method in the above-mentioned patent has the following disadvantages: In order to obtain a large number of somatic cells, manual milking is carried out several hours after early milking, and EDTA is added after milk samples are collected, and gauze filtering is required. Separation of somatic cells is more cumbersome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The invention relates to an optimization process of milk somatic cell separation process, which includes the separation process of milk somatic cell precipitation process and the RNA extraction process.

[0040] The process of separating milk somatic cell precipitation includes the following steps:

[0041] S1: Use a 15mL centrifuge tube to collect fresh milk samples;

[0042] S2: The fresh milk squeezed out of each cow is packed into 4 or several 15ml centrifuge tubes, and centrifuged at 1800r / 10min / room temperature;

[0043] S3: Discard the whey and milk fat, and pipette with 1-2ml PBS in each tube to mix the sediment. After this step, all can be transferred to the same 15mL centrifuge tube and then centrifuged;

[0044] S4: Centrifuge at room temperature at 1500r for 5min, discard whey and milk fat, remove residual milk fat as much as possible, add 400 μL Trizol lysate to each tube, let it stand, and bring it back to the laboratory.

[0045] The main component of t...

Embodiment 2

[0067] A milk somatic cell separation process optimization process, the separation milk somatic cell precipitation process, comprises the following steps:

[0068] S1: Fresh milk squeezed from each cow is packed into two or more 50ml centrifuge tubes, centrifuged at 1900g / 14min / room temperature;

[0069] S2: Discard the whey and milk fat, pipette and mix the precipitate with 1-2ml PBS in each tube;

[0070] S3: Transfer the mixed solution of all 50ml centrifuge tubes to a 15ml centrifuge tube;

[0071] S4: Centrifuge at 1900g room temperature for 10min, discard whey and milk fat, remove residual milk fat as much as possible, add 1mL Trizol lysate, let stand and bring back to the laboratory.

[0072] The specific operation in S4 is to put dry ice into the dry ice after standing at room temperature for 15 minutes, and then bring it back to the laboratory.

[0073] The RNA extraction process comprises the steps of:

[0074] S11: using the AM1561 kit;

[0075] S12: transfer th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a milk somatic cell separation process optimization process, and relates to the technical field of milk somatic cell RNA extraction. The milk somatic cell separation process optimization process comprises a milk somatic cell separation precipitation process and an RNA extraction process, wherein the milk somatic cell separation precipitation process comprises the following steps of: collecting a fresh milk sample by using 15mL centrifuge tubes; respectively filling the fresh milk extruded from each cow into four or a plurality of 15mL centrifuge tubes, and centrifuging at 1800r / 10 min / room temperature; and discarding whey and milk fat; and the RNA extraction process comprises the following steps: adopting an AM1561 kit; and transferring lysate in the 15mL centrifuge tubes to a 1.5mL centrifuge tube. According to the milk somatic cell separation process optimization process disclosed by the invention, milk somatic cells are separated on the spot without filtering or adding additional reagents, so that the time is greatly saved, unnecessary operations are reduced, the time is saved, the operations are simplified, and the milk somatic cell separation precipitation process is quicker, simpler and more convenient.

Description

technical field [0001] The invention relates to the technical field of milk somatic cell RNA extraction, in particular to an optimization process of milk somatic cell separation process. Background technique [0002] There are two sources of somatic cells in milk, one is from the epithelial cells in the secretory tissue of the mammary gland, also known as glandular cells; the other is from white blood cells that die when fighting inflammation, glandular cells are normal somatic cells, and are the metabolic process of the mammary gland The content of white blood cells in milk is relatively constant, and white blood cells are a kind of defense cells that can kill germs that infect mammary glands and repair damaged tissues. Therefore, the number of white blood cells in milk varies with the physiological state and health level of dairy cows. There are great changes, and the optimization of milk somatic cell isolation process is the optimization of milk somatic cell RNA extractio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/078C12N15/10
CPCC12N5/0631C12N5/0634C12N15/1017C12N2509/10C12Q2523/32
Inventor 郭勇庆韩印如邓铭柳广斌李耀坤孙宝丽刘德武张家豪魏子维
Owner SOUTH CHINA AGRI UNIV