A method, device and storage medium for diploid assembly
A diploid and haplotype technology, applied in the fields of genomics, proteomics, instruments, etc., can solve the problems of limited application prospects, typing impact, and high genomic heterozygosity, and achieve the effect of overcoming difficulties in obtaining
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[0085] In this case, a diploid assembly test was performed on the F1 generation high-heterozygous genome of hybrid maize. The parents of the hybrid maize were maize B73 and maize SK, respectively. The diploid genome size is about 4.5Gb. In this experiment, this example was based on PacBio HiFi data, Hi-C data, and gamete single-cell data to perform diploid assembly of this hybrid F1 individual. details as follows:
[0086] 1. Construction of the initial genome
[0087] In this example, the PacBio HiFi data of hybrid F1 individuals were obtained based on the parental genome sequence simulation. The genomic data of the parents are published public data.
[0088] The SK genome download address in this example is:
[0089] https: / / db.cngb.org / search / assembly / CNA0002536 / ;
[0090] The B73 genome download address is:
[0091] ftp: / / ftp.ensemblgenomes.org / pub / plants / release-50 / fasta / zea_mays / dna / ;
[0092] The download address of pbsim software is: https: / / github.com / pfaucon / P...
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