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A kind of Saccharomyces cerevisiae and method for improving the production of γ-aminobutyric acid by Saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae and aminobutyric acid, applied in the field of microbial fermentation, can solve the problem of low GABA production

Active Publication Date: 2022-07-15
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide Saccharomyces cerevisiae SC-125 (preserved at China Microorganism Culture Collection Management Committee on November 26, 2020) isolated, cultured and identified from naturally fermented fruit samples. Microbiology Center, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, named Saccharomyces cerevisiae, and the preservation number is CGMCC 21236), and the plant milk that has a symbiotic effect with the yeast strain Bacillus M912 (preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on November 26, 2020, the address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, classified as plant milk Bacillus Lactobacillus plantarum, preservation number is CGMCC 21237), by adding Lactobacillus plantarum M912 cell-free fermentation supernatant to GABA-producing Saccharomyces cerevisiae SC-125 fermentation medium, to increase GABA production and improve single Saccharomyces cerevisiae SC-125 fermentation synthesis The problem of low GABA production

Method used

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  • A kind of Saccharomyces cerevisiae and method for improving the production of γ-aminobutyric acid by Saccharomyces cerevisiae
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  • A kind of Saccharomyces cerevisiae and method for improving the production of γ-aminobutyric acid by Saccharomyces cerevisiae

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Embodiment 1

[0026] (1) Preparation of Lactobacillus plantarum M912 cell-free fermentation supernatant: Lactobacillus plantarum M912 was activated in MRS liquid medium, cultivated at 30°C for 12h to logarithmic growth phase, and the bacterial concentration was 10 7 CFU / mL. 10000g, centrifuged at 4°C for 10min, collected the supernatant, and filtered with a 0.22 μm membrane to obtain a cell-free supernatant.

[0027] (2) Preparation of GABA-producing Saccharomyces cerevisiae SC-125 seed culture: γ-aminobutyric acid-producing GABA-producing Saccharomyces cerevisiae SC-125 was activated in YPD medium containing 5 g / L L-sodium glutamate, and cultured at 30°C To the logarithmic growth phase, the bacterial concentration is 10 7 CFU / mL.

[0028] (3) GABA-producing Saccharomyces cerevisiae SC-125 fermented to synthesize GABA: inoculate GABA-producing Saccharomyces cerevisiae SC-125 with 5% of the inoculum in YPD liquid medium containing 6g / LL-sodium glutamate, add 8 % (v / v) of Lactobacillus pla...

Embodiment 2

[0031] (1) Preparation of Lactobacillus plantarum M912 cell-free fermentation supernatant: Lactobacillus plantarum M912 was activated in MRS liquid medium, cultivated at 30°C for 12h to logarithmic growth phase, and the bacterial concentration was 10 7 CFU / mL. 10000g, centrifuged at 4°C for 10min, collected the supernatant, and filtered with a 0.22 μm membrane to obtain a cell-free supernatant.

[0032] (2) Preparation of GABA-producing Saccharomyces cerevisiae SC-125 seed culture: γ-aminobutyric acid-producing GABA-producing Saccharomyces cerevisiae SC-125 was activated in YPD liquid medium containing 5 g / L L-glutamate, at 30°C Cultivated to the logarithmic growth phase, the bacterial concentration was 10 7 CFU / mL.

[0033] (3) GABA-producing Saccharomyces cerevisiae SC-125 fermented to synthesize GABA: inoculate GABA-producing Saccharomyces cerevisiae SC-125 with 5% of the inoculum in MRS liquid medium containing 12g / L L-sodium glutamate, add YPD liquid medium 9% (v / v) of...

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Abstract

The invention provides a Saccharomyces cerevisiae and a method for improving the production of γ-aminobutyric acid by the Saccharomyces cerevisiae. The GABA-producing Saccharomyces cerevisiae is obtained by culturing and screening as a fermentation microorganism, and the cell-free fermentation supernatant of Lactobacillus plantarum M912 is exogenously added to increase the GABA yield. . By adopting the method of the present invention, the concentration of GABA obtained is 3.96-7.68g / L; the concentration of GABA obtained by fermentation of GABA-producing Saccharomyces cerevisiae SC-125 in the control group (no cell-free supernatant is added to the fermentation medium) is 1.24-1.60g / L L. Compared with the control group, GABA production was increased by 3.19-4.80 times.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a Saccharomyces cerevisiae and a method for improving the production of γ-aminobutyric acid by the Saccharomyces cerevisiae. Background technique [0002] γ-aminobutyric acid (GABA) is a non-protein amino acid, which acts as an important neuroinhibitory transmitter in mammalian brain tissue, and has the functions of sedating nerves, improving sleep, regulating hormone secretion, and improving brain function. function and other important physiological activities. Due to the effective physiological function of GABA, it is a safe, low-cost and effective method to screen and obtain safe and functional microbial strains producing GABA from nature by using the microbial fermentation synthesis method, and to use its fermentation to prepare fermented products rich in GABA. [0003] As a recognized food-grade safe microorganism, yeast is an important starter in fermented f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12P13/00C12P39/00C12R1/865C12R1/25
CPCC12P13/005C12P39/00
Inventor 张庆唐洁向文良孙向阳汪杰吴敏张恕铭雷丹李婵媛郑淼心
Owner XIHUA UNIV
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