Flower bud stem cells and extraction method thereof, and flower bud stem cell essence

An extraction method and stem cell technology, applied in the field of plant stem cells, can solve the problem of lack of gardenia stem cells lotus stem cells and sea fennel stem cells extraction and culture methods, etc., and achieve the effects of short culture period, skin lubrication, and wrinkle reduction.

Pending Publication Date: 2021-05-28
深圳欧珈再生医学抗衰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a flower bud stem cell and its extraction method, and a flower bud stem cell essence, aiming at solving the lack of high-efficiency gardenia stem cells, lilac stem cells, lotus stem cells and seaweed stem cells in the prior art. Problems with the extraction and culture methods of fennel stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] A flower bud stem cell, extracted and cultivated by the following steps:

[0084] A. Collect gardenia flower buds, lilac flower buds, lotus flower buds and sea fennel flower buds, clean them, then sterilize their surfaces, and select three relatively healthy petals from each petal to extract stem cells;

[0085] B, gardenia flower bud, lilac flower bud, lotus flower bud and sea fennel flower bud are cut into long 3mm, wide 3mm, high 2mm profile shape, and all contain cambium in each piece;

[0086] C. Treat each prepared flower bud with a high-pressure osmotic solution to lose the vitality of the cortex in the differentiated tissue, and wherein the cambium remains alive;

[0087] D. Inoculate the flower buds after high-pressure infiltration into an induction medium with a pH value of 5, and culture them under low light for 3 days at 22°C to grow callus; wherein, the induction medium contains MS, 2.0mg / Semi-solid medium of L NAA, 3.0 mg / L 6-BA;

[0088] E. After the c...

Embodiment 2

[0090] A flower bud stem cell is extracted and cultivated by the following steps:

[0091] A. Collect gardenia flower buds, lilac flower buds, lotus flower buds and sea fennel flower buds, clean them, then sterilize their surfaces, and select three relatively healthy petals from each petal to extract stem cells;

[0092] B, gardenia flower bud, lilac flower bud, lotus flower bud and sea fennel flower bud are cut into long 5mm, wide 4mm, high 3mm profile shape, and all contain cambium in each sheet;

[0093] C. Treating each prepared flower bud with a high-pressure osmotic solution to lose the vitality of the cortex in the differentiated tissue, and wherein the cambium remains alive;

[0094] D. Inoculate the flower buds after high-pressure infiltration into an induction medium with a pH value of 6, and culture them under low light for 5 days at 25°C to grow callus; wherein, the induction medium contains MS, 5.0mg / Semi-solid medium of L NAA, 5.0mg / L 6-BA;

[0095] E. After t...

Embodiment 3

[0097] A flower bud stem cell is extracted and cultivated by the following steps:

[0098] A. Collect gardenia flower buds, lilac flower buds, lotus flower buds and sea fennel flower buds, clean them, then sterilize their surfaces, and select three relatively healthy petals from each petal to extract stem cells;

[0099] B, gardenia flower bud, lilac flower bud, lotus flower bud and sea fennel flower bud are cut into long 6mm, wide 5mm, high 6mm shape, and all contain cambium in each piece;

[0100] C. Treating each prepared flower bud with a high-pressure osmotic solution to lose the vitality of the cortex in the differentiated tissue, and wherein the cambium remains alive;

[0101] D. Inoculate the flower buds after high-pressure infiltration into an induction medium with a pH value of 7, and culture them under low light for 8 days at 27°C to grow callus; wherein, the induction medium contains MS, 10mg / L The semi-solid medium of NAA, 0.10 mg / L 6-BA;

[0102] E. After the cal...

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PUM

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Abstract

The invention discloses a flower bud stem cell and an extraction method thereof, and a flower bud stem cell essence. The extraction method comprises the following steps: A, collecting gardenia flower buds, lilac flower buds, lotus flower buds and crithmum maritimum flower buds, cleaning the collected flower buds, disinfecting and sterilizing the surfaces of the flower buds, and respectively selecting three healthy petals in each bud to extract stem cells; B, cutting the gardenia flower buds, the lilac flower buds, the lotus flower buds and the crithmum maritimum flower buds into shapes with a length of 3 mm, a width of 3 mm and a height of 2 mm, wherein each piece contains a cambium; and C, treating each prepared flower bud by using a high-pressure penetrating fluid so as to allow cortex in differentiated tissue to be inactivated, wherein the cambium is kept active. The extraction method disclosed by the invention is short in culture period and high in yield and active ingredient content; the flower bud stem cells can reduce wrinkles; and the flower bud stem cell essence disclosed by the invention can effectively reduce wrinkles and make skin smoother.

Description

technical field [0001] The invention relates to the technical field of plant stem cells, in particular to a flower bud stem cell, an extraction method, and a flower bud stem cell essence. Background technique [0002] Plant stem cells (plant stem cells) are undifferentiated cells with strong self-renewal and continuous division capabilities. Botanists have discovered that plant stem cells exist in flower buds, and call them meristematic cells (bud stem cells). Plant meristem cells will be activated with tissue creation, perform self-repair functions, and regenerate damaged tissues. Fragile flower buds lack hard epidermis protection and are often exposed to environmental aggression. Because of this, the meristem cells in flower buds must produce high concentrations of phenylpropanoids (phenyl pro panoids), which are effective against environmental pollution and repair cell damage. Studies have found that the regeneration capacity of flower bud stem cells is 1000 times that o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A61K8/99A61K8/9794A61K8/9789A61Q19/00A61Q19/08
CPCC12N5/04C12N5/0025A61K8/99A61K8/9794A61K8/9789A61Q19/00A61Q19/08
Inventor 曾杰
Owner 深圳欧珈再生医学抗衰生物工程有限公司
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