Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing human umbilical cord-derived Muse cells

A culture method and technology of human umbilical cord, applied in the field of cell research, can solve the problems of low efficiency and low content of Muse cells, and achieve the effect of strong proliferation ability and reasonable design

Inactive Publication Date: 2021-05-28
HENAN YINFENG BIOENG CO LTD +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it has been found that Muse cells exist in mesenchymal tissues such as bone marrow, fat, skin, spleen, pancreas, and umbilical cord. The commonly used screening sources are bone marrow mesenchymal and adipose mesenchymal tissues, but the content of Muse cells is relatively small, and they will be expanded later. The efficiency of multiplication is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing human umbilical cord-derived Muse cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] see figure 1 , a culture method of human umbilical cord derived Muse cells, comprising the following steps:

[0023] Include the following steps:

[0024] Step 1. Identify the participants.

[0025] Step 2: Determine the experimental equipment and reagents.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture method of human umbilical cord-derived Muse cells. The culture method comprises the following steps: determining participants, determining experimental instruments and reagents, determining an experimental method, separating the Muse cells, and performing in-vitro amplification culture on the Muse cells. The method has the beneficial effects that by applying the culture system, the MSCs obtained by separation are in a typical fibroblast form and have relatively strong multiplication capacity. According to the Muse cell culture method disclosed by the invention, the Muse cells are in positive expression, CD29, CD44, CD90 and CD105 are all in positive expression, and CD34 and CD45 are in negative expression, so that the Muse cells with relatively high purity can be obtained by the culture method, and a certain reference is provided for clinical application of the Muse cells in mesenchymal stem cell treatment, tissue regeneration, immunoregulation and the like in future.

Description

technical field [0001] The invention relates to a method for cultivating Muse cells derived from human umbilical cord, in particular to a method for cultivating Muse cells derived from human umbilical cord, and belongs to the technical field of cell research. Background technique [0002] Multilineage differentiating stress enduring (Muse) cells are a special kind of adult pluripotent stem cells. In 2010, Mari Dezawa et al. isolated multilineage differentiated continuous stress cell Muse from BMSCs and skin fibroblasts, which specifically expressed stage-specific embryonic antigen-3 (stagespecific embryonic antigen-3, SSEA3) and CD105, which can Cells that differentiate into the 3 germ layers are an ideal source of potential seed cells. Muse cells are a subpopulation of mesenchymal stromal cells (MSCs). Muse cells have been found to exist in mesenchymal tissues such as bone marrow, fat, skin, spleen, pancreas, and umbilical cord. The commonly used screening sources are Bon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2509/00C12N2509/10C12N2533/90
Inventor 邱孟广郭晓丹崔兴涛李欢欢
Owner HENAN YINFENG BIOENG CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com