Procalcitonin chemiluminescence immunodetection kit

A technology of chemiluminescent immunity and detection kits, which is applied in the fields of chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc. Poor cartridge specificity and other issues, to save manpower and time, improve shelf life and accuracy, simple operation and time-saving effects

Active Publication Date: 2021-06-04
宁波海尔施智造有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the sensitivity has improved (0.008ng / ml), the specificity of the kit is poor and the detection range is average (0-100ng / mL)
Another Chinese patent CN102359958B discloses a PCT chemiluminescence detection kit, which uses magnetic particle-coupled antibody as a fixed separation phase, a biotin-streptavidin multistage amplification system, and horseradish peroxidase as the The catalytic enzyme catalyzes the luminescent substrate to emit light, but its detection sensitivity is only 0.05ng / mL

Method used

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  • Procalcitonin chemiluminescence immunodetection kit
  • Procalcitonin chemiluminescence immunodetection kit
  • Procalcitonin chemiluminescence immunodetection kit

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Embodiment 1

[0064] The kit of this embodiment includes a magnetic separation reagent, a first reagent, a second reagent and a procalcitonin series calibrator.

[0065] The preparation method of magnetic separation reagent is:

[0066] Resuspend the magnetic particles with 0.05mol / L, pH 8.0 boric acid buffer, the concentration of the magnetic particles after resuspension is 6mg / ml; The ratio is 80:1, after mixing, add 1mol / L ammonium sulfate solution, the volume added is 1 / 2 of the sum of the volume of magnetic particles and PCT monoclonal antibody, suspend and react at 25°C for 8 hours; perform magnetic separation after the reaction , Remove the supernatant, add the blocking agent CE210 to the precipitate, suspend and react at 25°C for 1 hour, add 250 μL of blocking agent CE210 per 10 mg magnetic beads; then wash 3 times with Tris buffer (pH 7.4) containing 1% Tween20 , and finally add tris buffer containing 0.06wt% bovine serum albumin, 0.007mol / L, pH 7.4, so that the concentration of m...

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Abstract

The invention provides a procalcitonin chemiluminescence immunodetection kit. The kit comprises a magnetic separation reagent, a first reagent and a second reagent. The magnetic separation reagent contains a magnetic particle reagent coated with a procalcitonin monoclonal antibody; the first reagent contains a biotin-labeled procalcitonin monoclonal antibody solution; and the second reagent contains a streptavidin-labeled alkaline phosphatase solution. The PCT kit provided by the invention has relatively low PCT detection sensitivity and relatively wide PCT detection range.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection and relates to a procalcitonin chemiluminescent immunoassay kit. Background technique [0002] Bacteria or viruses invade through wounds, respiratory tract, urinary tract, and digestive tract. In severe trauma, decreased systemic resistance, imbalance of normal flora in the body, and translocation of bacteria, it can cause severe infection symptoms in the body, which is the cause of clinical symptoms. Various complications, such as sepsis, septic shock, multiple organ dysfunction syndrome (MODS), and even the main cause of death. According to the statistics of the United States in 2001, there were 751,000 cases of severe sepsis alone in that year, and the average mortality rate was as high as 28.5%, which means that the number of deaths in that year was nearly three times that of AIDS (acquiredimmuneficiency syndrome, AIDS). Early diagnosis and timely treatment of infectious diseases a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N33/543G01N33/58G01N21/76
CPCG01N33/74G01N33/577G01N33/54326G01N33/581G01N21/76G01N2333/585
Inventor 柳建敏王薇汪云峰
Owner 宁波海尔施智造有限公司
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