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Preparation method of pineapple tissue culture seedlings

A technology of tissue culture and pineapple, applied in the direction of botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of high speed, small quantity, and inability to meet large-scale replacement of pineapple varieties, etc., to improve reproduction Coefficient, promotional effect

Active Publication Date: 2021-06-11
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Large-scale variety replacement requires enough new varieties and high-quality seedlings to rely on; while the number of sucking buds, crown buds, and descending buds is small and the rate is high. Obviously, it cannot meet the needs of large-scale replacement of pineapple varieties

Method used

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  • Preparation method of pineapple tissue culture seedlings
  • Preparation method of pineapple tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of pineapple tissue culture seedling in the present embodiment, comprises the steps:

[0042] 1) Select pineapple varieties Bali (Comte de Paris) and Tai Nong No. 4;

[0043] Select pineapple plants 4 to 6 months after inflorescence pumping, pick crown buds, descending buds, sucking buds, or tuber buds of these plants, peel off the leaves, and dip in 1% detergent with a soft brush. Clean, rinse the stem section with tap water, and remove the attachments on the surface of the stem section.

[0044] 2) Spray the above-mentioned stem section with 75% alcohol on its surface, then turn to the ultra-clean workbench, in the ultra-clean workbench, put the stem section into 75% alcohol and soak for 1 minute, then take out the stem section, and use Rinse 5 times with sterile water, put into 0.1% mercuric chloride dripped with 2 drops of Tween20 and soak for 1 minute, then rinse 5 times with sterile water, and transfer the stem section to a petri dish with ...

Embodiment 2

[0060] The preparation method of pineapple tissue culture seedling in the present embodiment selects crown buds, descending buds, sucking buds or tuber buds of pineapple varieties Golden Diamond, Sweet Honey and Golden Pineapple to prepare tissue culture seedlings. All the other preparation processes are the same as in Example 1.

[0061] In this example, Jinzuan and Tianmi can obtain 3000±31 tissue-cultured seedlings from one crown bud, 2000±59 tissue-cultured seedlings from one sucking bud, and 1500±66 tissue-cultured seedlings from one seedling bud. For seedlings, 1000±72 tissue cultured seedlings can be obtained from one tuber bud. The reproduction coefficients of crown buds, suction buds, descendant buds and tuber buds were 3000, 2000, 1500 and 1000, respectively. There were no significant differences between the Golden Diamond and Sweet Honey varieties. The tissue culture seedlings are in good health.

[0062] One crown bud of golden pineapple can obtain 2500±89 tissu...

Embodiment 3

[0064] In the preparation method of pineapple tissue culture seedlings in the present embodiment, crown buds, descending buds, sucking buds, or tuber buds of pineapple varieties Victoria and watermelon pineapple are selected to prepare tissue culture seedlings. All the other preparation processes are the same as in Example 1.

[0065] In this embodiment, one crown bud of Victoria and watermelon pineapple can obtain 3000 ± 31 tissue cultured seedlings, one suction bud can obtain 2000 ± 58 tissue cultured seedlings, and one seed bud can obtain 1500 ± 62 tissue cultured seedlings , one tuber bud can obtain 1000±72 tissue culture seedlings. The reproduction coefficients of crown buds, suction buds, descendant buds and tuber buds were 3000, 2000, 1500 and 1000, respectively. There were no significant differences between Victoria and watermelon pineapple varieties. The tissue culture seedlings are in good health.

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Abstract

The invention relates to a preparation method of pineapple tissue culture seedlings, and belongs to the technical field of pineapple tissue culture seedling preparation. The preparation method of the pineapple tissue culture seedlings comprises the steps of inducing buds on a pineapple plant to generate an embryogenic callus, then establishing an embryogenic suspension cell line, and regenerating by using the embryogenic suspension cell line to obtain the pineapple tissue culture seedlings. According to the preparation method of the pineapple tissue culture seedlings, the propagation coefficient is large, tens of thousands of independent cells can be obtained from one bud through the callus and the suspension cell line, and the independent cells can be independently developed into an independent plant under proper conditions; on the other hand, suspension cells are easy to preserve, and the preserved suspension cell line is applied to be quickly regenerated to generate corresponding seedlings when seedlings of a certain variety are needed, so that the culture time of the pineapple seedlings is shortened, and the sustainable supply of the pineapple seedlings is effectively improved.

Description

technical field [0001] The invention relates to a method for preparing pineapple tissue culture seedlings, and belongs to the technical field of pineapple tissue culture seedling preparation. Background technique [0002] Pineapple is the main tropical fruit in Hainan Province, and plays an important role in the economic development of Hainan Province. Hainan Province has abundant light and heat resources, and it has a unique advantage in developing the pineapple industry. At present, one of the main varieties of pineapple in Hainan Province is Bali, which is easy to promote flowering and easy to manage; but the quality is not high, the profit is low, and the time to market is concentrated. In recent years, there have been many unsalable situations. Therefore, pineapple growers have tended to replace Barry pineapples with new varieties with high economic benefits; obtaining enough high-quality seedlings of new pineapple varieties is the prerequisite for realizing the replac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 常胜合舒海燕李科明孙威许桂莺郭刚詹儒林
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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