Method for researching function of MDK gene in promoting proliferation of brain glioma and application of MDK gene

A brain glioma and glioma cell technology is applied in the molecular mechanism and early warning field of glioma proliferation caused by interfering with MDK gene expression, which can solve problems such as no research analysis and exploration, and achieve the promotion of tumor cell growth and survival, The effect of controlling tumor growth

Inactive Publication Date: 2021-06-11
南宁市第一人民医院
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  • Description
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Problems solved by technology

[0004] In summary, the existing problem is that we found that MDK can promote the proliferation of glioma cells, but there is no research analysis to explore its specific mechanism

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  • Method for researching function of MDK gene in promoting proliferation of brain glioma and application of MDK gene
  • Method for researching function of MDK gene in promoting proliferation of brain glioma and application of MDK gene
  • Method for researching function of MDK gene in promoting proliferation of brain glioma and application of MDK gene

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. The specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] In order to solve the technical problem that the specific mechanism of MDK affecting the proliferation of glioma is not yet clear, the present invention will be described in detail below in conjunction with specific analysis. The specific mechanism of MDK promoting glioma proliferation has not yet been clearly reported. Therefore, the present invention selects MDK gene as the main analysis content. The present invention preliminarily confirms that MDK is expressed in glioma cell lines. On this basis, the present invention intends to further clarify the specific mechanism of MDK affecting glioma proliferation through the following experiments: ...

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Abstract

The invention relates to a method for researching a function of promoting brain glioma proliferation by an MDK gene and application of the MDK gene. The method comprises the following steps: (1) analyzing MDK expression of a glioma cell line through RT-qPCR, (2) carrying out lentivirus-mediated overexpression and MDK knockout, (3) interfering the influence of MDK gene expression on glioma cell proliferation, and (4) analyzing and detecting the conditions of P-PI3K, PI3K, P-Akt, Akt, p-ERK, N-cadherin, vimentin and beta-catenin in the glioma cell line which interferes with MDK gene expression through Western blot. The PI3K / AKT pathway is a glioma cell proliferation mechanism mediated by the MDK. The invention provides a new thought for treating glioma, and provides a new way for researching glioma drug treatment targets.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a molecular mechanism and an early warning method for interfering with MDK gene expression to cause glioma proliferation. Background technique [0002] Gliomas are a heterogeneous group of brain tumors with unique biological and clinical features. Although advances have been made in surgical techniques and clinical treatment, the treatment of glioma is still challenging, and the treatment success rate and overall survival rate are still high, and the 5-year survival rate is only 5% [Ostrom QT, Gittleman H, Fulop J, et al. CBTRUS Statistical Report: Primary Brain and Central Nervous System Tumors Diagnosed in the United States in 2008-2012. Neuro Oncol 2015; 17 Suppl 4:iv1-iv62]. [0003] MDK (Midkine) is a protein-coding gene that encodes a heparin-binding growth factor associated with cancer development. MDK is overexpressed in most malignant tumors including gliobla...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12Q1/02C12Q1/6851C12Q1/6886G01N33/569G01N33/68
CPCC12N15/86G01N33/5005C07K14/475C12Q1/6851C12Q1/6886G01N33/56966G01N33/6854C12N2740/15043C12Q2600/158C12Q2531/113
Inventor 魏风胡北泉兰杰邹东华黄贤伦樊华声利丽
Owner 南宁市第一人民医院
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