Construction method and application of arvcf gene knockout animal model

Abstract

The invention relates to a method and application for establishing an Arvcf gene knockout mouse model, wherein the mouse model is a mouse that knocks out seven exons in the Arvcf gene, and discloses a method for establishing an Arvcf gene knockout mouse model. method of the mouse model. First determine the knockout region of the gene to be knocked out Arvcf, then design and synthesize specific sgRNAs and corresponding primers, then linearize and purify the constructed sgRNA / Cas9 vector for in vitro transcription, and finally inject together by microinjection The fertilized eggs of mice were transplanted into the uterus of pseudopregnant female mice to establish the Arvcf gene knockout mouse model. The tails of the obtained progeny mice were collected and DNA was extracted, and the obtained mice were confirmed to be wild-type or homozygous or heterozygous for the target region of the Arvcf gene by using two pairs of primers through two PCR reactions and sequencing. mice. This knockout mouse model can be used to study the role of ARVCF in the psychiatric diseases involved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a construction method and application of an Arvcf gene knockout animal model. Background technique [0002] The protein encoded by the ARVCF (Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome) gene is a member of the p120ctn catenin family, and other members include p120ctn, δ-catenin and p0071. This family of proteins plays a key role in the formation of the adhesion junction complex and also plays an important role in various aspects of neuromorphogenesis, and members of this family have also been found to be associated with a variety of neurodevelopmental diseases. In addition, the ARVCF protein is part of the cadherin-catenin complex and may regulate cadherin-mediated junctional structures and intercellular adhesion in various cell types, and the ARVCF gene has also been reported to be involved in neural pathways from the ganglionic eminence Meta migration...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a method and application for establishing an Arvcf gene knockout mouse model based on a new generation of gene editing technology, so as to solve the technical problems of the existing gene editing technology such as cumbersome operation and high cost, and at the same time, it can also solve the lack of Issues in Animal Models for Studying ARVCF Biological Function

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