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Multi-mode immune instant analysis method excited by nano titanium carbide hybrid

A nano-titanium carbide and hybrid technology, applied in the fields of biological material analysis, analytical materials, biological testing, etc., can solve the problems of limiting the popularization and promotion of ELISA, complicated steps, and high cost of biomarker detection, so as to improve the photothermal performance. Effect

Active Publication Date: 2021-06-18
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the high cost of biomarker detection, complicated steps, and the need for professional operators, the popularization and promotion of ELISA in remote areas is limited.

Method used

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  • Multi-mode immune instant analysis method excited by nano titanium carbide hybrid
  • Multi-mode immune instant analysis method excited by nano titanium carbide hybrid
  • Multi-mode immune instant analysis method excited by nano titanium carbide hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A multimodal immunological instant analysis method excited by nano-titanium carbide hybrids (such as figure 1 shown):

[0032] 1) Dissolve 0.5% polyvinyl alcohol PVA solid in 10 mL ultrapure water, add 2 mL glycerin and heat for 20 minutes under continuous stirring to obtain a uniform hydrosol;

[0033] 2) Then, the hydrosol was treated with 1 mL of 0.5 mol / L NaOH solution at 40°C for 1 h, and 1 mL of 1% cyanuric chloride (dissolved in 1,4-dioxane) was added to react 1 Hour. Then dripping mass fraction is in the acetic acid solution of 3% and gets rid of excess alkali;

[0034] 3) Subsequently, transfer the hydrosol to 100 μL per well in a 96-well plate, and cool to room temperature to obtain polyvinyl alcohol hydrogel as a flexible temperature-sensitive conductive device SED;

[0035] 4) Add 30 μL of 10% HCl to etch the surface of the flexible temperature-sensitive conductive device SED for 10 minutes and rinse it several times with a phosphate buffer solution of pH...

Embodiment 2

[0046] A multi-mode immune instant analysis method excited by nano-titanium carbide hybrids, the steps are as follows:

[0047] 1) Drop 30μL Leuco-MB chromogenic reagent on SED / Ab / Ag-Ti 3 C 2 React on @CuNC modified substrate for 90s to get SED / Ab / Ag-Ti 3 C 2 @CuNCs / MB modified substrate, SED / Ab / Ag-Ti with 808nm laser 3 C 2 @CuNCs / MB modified substrate for radiation;

[0048] 2) Measurement of human anti-asialoglycoprotein receptor human anti-ASGPR: SED / Ab / Ag-Ti at different concentrations collected by smartphone 3 C 2 @CuNCs / MB retouched the photo of the substrate, and converted it into red, green and blue RGB images with the professional drawing software PowerPoint, calculated 1×10 -6 U / mL–1×10 -2 Color values ​​between U / mL. The color change can be defined in the following way: Color value = Red value (R) + Green value (G) × 256 + Blue value (B) × 65536; use 808nm laser to SED / Ab / Ag-Ti 3 C 2 @CuNCs / Leuco-MB modified substrate radiation for 1min (optical po...

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Abstract

The invention discloses a multi-mode immune instant analysis method excited by a nano titanium carbide hybrid. The plasma nano hybrid Ti3C2-coated CuNCs has good photothermal conversion performance, and can cause the SED temperature rise of the flexible conductive substrate under the excitation of near-infrared light. Besides, the high catalyst activity of the Ti3C2-coated CuNCs can catalyze oxidation of a colorimetric reagent Lecuo-MB to generate blue methylene blue MB, so that the color of the substrate is changed from light blue to dark blue. The methylene blue MB also has excellent photo-thermal performance, and the synergistic effect of the Ti3C2-coated CuNCs and the photo-thermal effect of the MB further enhances light absorption and amplifies a temperature signal. Meanwhile, continuously increased heat also accelerates migration of charges in the SED, resistance change caused by temperature rise can be monitored in real time through a digital multimeter, and a temperature signal can be converted into an electric signal. According to the method, high-sensitivity and multi-signal immunodetection on the human anti-ASGPR is realized, the method has huge potential in clinical diagnosis of malignant diseases, and a new way is opened up for detection of a nursing point.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and in particular relates to a multimodal immunoassay for the detection of human anti-asialoglycoprotein receptor human anti-ASGPR by a photothermochemical sensor based on nano-titanium carbide hybrids method. Background technique [0002] Many malignant diseases seriously threaten public health due to their long incubation period, difficulty in early diagnosis, and lack of effective preventive measures. Therefore, it is essential to design reliable early monitoring methods to reduce the threat of malignant diseases to human health. In recent years, with the discovery of biomarkers, it has provided an important reference for the early diagnosis of various malignant tumors. However, the high cost of biomarker detection, complicated steps, and the need for professional operators limit the popularization and promotion of ELISA in remote areas. For the se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N33/531G01N33/53
CPCG01N33/5767G01N33/531G01N33/5302G01N2333/705Y02A50/30
Inventor 戴宏魏洁陈妍洁张书培皇怡甜任卉竹李秀华
Owner FUJIAN NORMAL UNIV
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