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Chenopodium quinoa willd hypocotyl in-vitro regeneration method

An in vitro regeneration and hypocotyl technology, applied in the field of plant tissue culture and biology, can solve the problems affecting the in-depth research of quinoa, the difficulty of genetic transformation of quinoa, the lack of an in vitro regeneration system of quinoa, etc., to simplify the steps of tissue culture , cost reduction, callus densification effect

Active Publication Date: 2021-06-25
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the difficulty of genetic transformation of quinoa seriously affects the in-depth study of quinoa, and the key factor restricting the genetic transformation of quinoa is the lack of in vitro regeneration system of quinoa

Method used

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  • Chenopodium quinoa willd hypocotyl in-vitro regeneration method
  • Chenopodium quinoa willd hypocotyl in-vitro regeneration method
  • Chenopodium quinoa willd hypocotyl in-vitro regeneration method

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Embodiment 1

[0032] (1) Materials: plump quinoa seeds;

[0033] (2) Disinfection: After the seeds are rinsed with sterile water, they are treated with 5‰ potassium permanganate solution for 3 minutes, then treated with 10% sodium hypochlorite solution for 15 minutes, rinsed with sterile water for 5 times, and the surface moisture of the material is absorbed by filter paper, and inoculated to MS In the basic medium, aseptic seedlings were obtained after culturing for 5 days, when the cotyledons had just developed ( figure 1 ). The amount of sucrose contained in the medium is 30g / L, the amount of agar powder contained is 5.5g / L, inositol is 0.1g / L, and the pH value of the medium is adjusted to 5.8; the culture condition is 25±2°C, and the light intensity 2000-3000lx, light 12h / d. figure 1 Shown is that the aseptic seedling stage when the present invention cuts the explant, that is, the cotyledon has just begun to cut in this period, and as a contrast, provides the aseptic seedling growth...

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Abstract

The invention discloses a chenopodium quinoa willd hypocotyl in-vitro regeneration system. Chenopodium quinoa willd hypocotyl is used as a starting material, a large number of adventitious buds can be obtained through induction, regenerated plants can be obtained without being limited by seasons, and technical support is provided for chenopodium quinoa willd transgenosis. A chenopodium quinoa willd hypocotyl in-vitro regeneration method provides technical guarantee for new chenopodium quinoa variety creation, molecular mechanism research and the like, and promotes the development of the chenopodium quinoa industry.

Description

technical field [0001] The invention relates to the field of plant tissue culture and biotechnology, in particular to a method for in vitro regeneration of quinoa hypocotyls. Background technique [0002] Quinoa (C.quinoa Wild, 2n=36) belongs to Amaranthaceae (Amaranthaceae), Chenopodioideae (Chenopodioideae) and Chenopodium (Chenopodium), an annual dicotyledonous plant. Its grain is used as food and has rich nutrients. Quinoa, known as the "Mother of Food", was selected by NASA as a staple food for space in the 1970s (Giusti L.Elgenero Chenopodium en Argentina 1:Numeros decromosomas.Darwiniana,1970,16:98-105.) . Biologically, quinoa exhibits outstanding stress resistance and adaptability, and has excellent cold and salt resistance. It is a star stress-resistant plant (Yang Farong, Liu Wenyu, Huang Jie, Wei Yuming, Jin Qian. Different quinoa varieties are Physiological responses to salt stress and evaluation of salt tolerance [J]. Journal of Herbal Medicine, 2017, 26(12): ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15
CPCA01H4/001A01H4/008A01G24/28A01G24/15
Inventor 刘洁玉朱木兰朱健康
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI