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Nano composite system with nitric oxide/photodynamic synergistic antibacterial and anti-inflammatory effects as well as preparation method and application of nano composite system

An anti-inflammatory effect, nitric oxide technology, applied in the field of nanocomposite system and its preparation, can solve the problem of less synergistic treatment, and achieve the effect of photodynamic high-efficiency therapeutic effect

Active Publication Date: 2021-06-29
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a variety of NO or PDT nanosystems have been disclosed, there are few reports on the synergistic treatment of the two

Method used

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  • Nano composite system with nitric oxide/photodynamic synergistic antibacterial and anti-inflammatory effects as well as preparation method and application of nano composite system
  • Nano composite system with nitric oxide/photodynamic synergistic antibacterial and anti-inflammatory effects as well as preparation method and application of nano composite system
  • Nano composite system with nitric oxide/photodynamic synergistic antibacterial and anti-inflammatory effects as well as preparation method and application of nano composite system

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Experimental program
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Effect test

Embodiment 1

[0044] The synthesis route of the nanocomposite system with nitric oxide / photodynamic synergistic antibacterial and anti-inflammatory effects is as follows: figure 1 As shown, it specifically includes the following steps:

[0045] (1) Preparation of UCNPs

[0046] 1mmol of RE a Cl 3 ·6H 2 O (RE=50%Y+49.5%Yb+0.5%Tm) was added into a three-neck round bottom flask containing 15mL ODE and 10mL OA, heated to 150°C with continuous stirring until a clear solution formed. After the reaction solution was cooled to room temperature, 4 mL of NH 4 Methanol solution of F (4mmol) and NaOH (2.5mmol), continued to react for 1 hour, then heated the mixture to 120°C, reacted for 30min, completely removed methanol and produced water. Then, under the protection of nitrogen flow, the temperature of the reactant was rapidly raised to 300° C., and the reaction was kept for 2 hours. Finally, the obtained pale yellow transparent reaction mixture was cooled to 60°C, and a sufficient amount of eth...

Embodiment 2

[0057] Bacterial keratitis treatment experiment

[0058] Choose a concentration of 10 7 CFU / mL of S.aureus to establish bacterial keratitis animal model. Use a disposable scalpel to scrape off the corneal epithelium in the central area of ​​the rat's right eye cornea about 3*3mm, drop 10 μL of bacterial solution to fully cover the epithelial defect area, and then close the upper and lower eyelids of the rat to allow the bacterial solution to stay for a sufficient time. Slit lamp examination and corneal thickness examination were carried out 24 hours after modeling, and rats with similar degree of inflammation were selected as the objects of subsequent animal experiments. 20 rats with successful modeling were selected and randomly divided into 5 groups (control group, UCNPs@SiO 2 、UCNPs@SiO 2 -AIE, UCNPs@SiO 2 -NO and UCNANs), 4 animals in each group. According to the grouping, the corresponding materials were dripped into the right eyes of the rats in the form of eye drop...

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Abstract

The invention discloses a nano composite system with nitric oxide / photodynamic synergistic antibacterial and anti-inflammatory effects as well as a preparation method and application of the nano composite system. The nano composite system comprises up-conversion nanoparticles of a core-shell structure, mesoporous silica wrapping the up-conversion nanoparticles, an AIE photosensitizer, an NO donor and modified polyethylene glycol which are loaded on the mesoporous silica. According to the nano composite system, the purpose of the synergy of two treatment modes triggered by one laser is achieved, the generated active oxygen, nitric oxide and peroxynitrite anions can be efficiently utilized to kill bacteria while the high biological safety is maintained, and the excellent antibacterial and anti-inflammatory effects are provided in the treatment of the S.aures infected bacterial keratitis model, and therefore the nano composite system has the potential of becoming an alternative strategy for treating bacterial keratitis.

Description

technical field [0001] The invention relates to the field of new materials, in particular to a nanocomposite system with nitric oxide / photodynamic synergistic antibacterial and anti-inflammatory effects, its preparation method and application. Background technique [0002] Bacterial keratitis is an inflammation of the cornea caused by bacterial infection. If left untreated, it can easily lead to corneal perforation, intraocular infection and even blindness. Currently, topical broad-spectrum antibiotics (such as levofloxacin, moxifloxacin, etc.) are the first-line therapy for the clinical treatment of bacterial keratitis. However, the efficacy of such drugs is declining as bacteria become increasingly resistant to traditional antibiotics. In addition, traditional antibiotic treatment is often unable to control excessive local inflammatory reactions, and even if the bacteria in the cornea are eliminated, about 50% of patients still suffer from visual impairment. Therefore, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/18A61K47/04A61P31/04A61P31/02A61P17/02A61P29/00B82Y5/00B82Y40/00A61K31/37
CPCA61K31/37A61K41/0057A61K47/02A61K47/186A61P17/02A61P29/00A61P31/02A61P31/04B82Y5/00B82Y40/00A61K2300/00
Inventor 张衡瑞王佰亮秦楠褚晓莹高强朱康宁曹小文杨梅
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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