A method for cultivating ginseng stem cells using a bioreactor

The technology of a biological reaction device and a culture method is applied in the field of cultivating ginseng stem cells by using a biological reaction device, which can solve the problems of limited division ability, weak resistance to stress, and degeneration of cell lines, etc., to achieve increased content, uniform and sufficient ventilation, and growth fast effect

Active Publication Date: 2022-06-28
SHANDONG ANRAN NANOMETRE IND DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of cell essence comes from differentiated somatic cells, so the ability to divide is limited and the ability to resist stress is not strong
In industrial production, cell line degeneration is prone to occur, and the ability to divide is weak

Method used

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  • A method for cultivating ginseng stem cells using a bioreactor
  • A method for cultivating ginseng stem cells using a bioreactor
  • A method for cultivating ginseng stem cells using a bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Embodiment 1 one-step method cultivates adventitious root of ginseng

[0143] (1) Induction of adventitious roots

[0144] The 20-year-old wild ginseng, removed the reed head, the stern part, the main root after cleaning, disinfection and sterilization, was cut into thin slices with a width of 0.6cm, a length of 0.7cm and a thickness of 0.3mm, and inoculated into the induction medium at 22±1°C. Under the condition of dark culture for 4 to 5 weeks, the adventitious roots of ginseng were induced; wherein, the induction medium included 4mg / L naphthalene acetic acid, 0.6mg / L gibberellin, 0.4mg / L kinetin, 0.1g / L citric acid, 0.05g / L L ascorbic acid, 30 g / L sucrose, 3 g / L phytogel, 1.55 g / L B5 medium and 1.21 g / L WPM medium, pH 5.8.

[0145] (2) Subculture of adventitious roots

[0146] Inoculate the adventitious root of the wild ginseng obtained in step (1) into the same induction medium as in step (1), and cultivate in the dark for 4 to 5 weeks under the same conditions; ...

Embodiment 2 1

[0150] Embodiment 2 one-step method cultivates adventitious root of ginseng

[0151] (1) Induction of adventitious roots

[0152] After washing, sterilizing and sterilizing the head of the 6-year-old garden ginseng, cut into slices with a width of 0.5cm, a length of 0.6cm and a thickness of 0.3mm, inoculated into the induction medium, and cultivated in the dark at 22±1°C for 4-5 days. Adventitious roots were induced every week; wherein, the induction medium included 6mg / L naphthalene acetic acid, 0.2mg / L gibberellin, 0.4mg / L kinetin, 1.2g / L citric acid, 0.1g / L ascorbic acid, 20g / L sucrose, 5g / L phytogel, 4g / L B5 medium and 1.8g / L WPM medium, pH 5.6.

[0153] (2) Subculture of adventitious roots

[0154] The adventitious roots obtained in step (1) are inoculated into the same induction medium as in step (1), and cultured in the dark for 4-5 weeks under the same conditions;

[0155] (3) Cultivation of adventitious roots

[0156] The adventitious roots obtained in step (2) ar...

Embodiment 3

[0157] Similar to the result of Example 1, adventitious roots can be induced in one step in the step (1) of the present embodiment, and the number of adventitious roots in Example 3 is one-step method to cultivate adventitious roots of ginseng

[0158] (1) Induction of adventitious roots

[0159] After washing, sterilizing and sterilizing the stems of 10-year-old ginseng under the forest, cut into slices with a width of 0.7cm, a length of 0.7cm and a thickness of 0.5mm, inoculated into the induction medium, and cultivated in the dark at 22±1℃ for 4~ Induce adventitious roots in 5 weeks; wherein, the induction medium includes 5mg / L naphthalene acetic acid, 1mg / L gibberellin, 0.1mg / L kinetin, 0.075g / L citric acid, 0.03g / L ascorbic acid, 40g / L sucrose, 4g / L phytogel, 2g / L B5 medium and 1g / L WPM medium, pH 6.0.

[0160] (2) Subculture of adventitious roots

[0161] The adventitious roots obtained in step (1) are inoculated into the same induction medium as in step (1), and cultu...

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Abstract

The invention discloses a method for cultivating ginseng stem cells by using a biological reaction device, which comprises the following steps: (1) adopting a one-step method to cultivate ginseng adventitious roots; (2) taking the root tip part of ginseng adventitious roots and placing them in an osmotic agent solution for treatment; 3) put the treated ginseng adventitious root root tip into the stem cell induction medium for culture, and then transfer to the stem cell expansion solid medium for culture; (4) collect the ginseng stem cells obtained in step (3), and inoculate them into the culture medium containing In the biological reaction device of the stem cell expansion liquid medium, culture in the dark to obtain ginseng stem cells. The present invention obtains a method for obtaining adventitious roots from mature ginseng and further obtaining ginseng stem cells with shorter required time and simpler steps, adopts a suitable biological reaction device for expanded culture, and has a high growth factor of stem cells and a high content of active ingredients in the stem cells.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for culturing ginseng stem cells using a biological reaction device. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is an Araliaceae ginseng plant, distributed in China, Japan, and Korea. Its rhizomes are valuable Chinese medicinal materials, known as "the king of herbs". Ginseng is sweet, slightly bitter, slightly warm, and has the functions of invigorating vitality, strengthening the pulse, invigorating the spleen and benefiting the lung, promoting body fluid and nourishing blood, soothing the mind and nourishing wisdom, etc. Wound thirst, palpitations, insomnia, etc. Ginsenoside is the main active ingredient of ginseng, which has the functions of anti-fatigue, anti-aging, regulating the central nervous system, improving immunity, improving cardiovascular and cerebrovascular insufficiency, and inhibiting tumor cell production. In recent y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00C12M3/00C12M1/04C12M1/12C12M1/00
CPCC12N5/04C12N5/0025A01H4/005A01H4/001C12M23/02C12M29/04C12M29/20C12M23/48C12M23/22C12M23/38
Inventor 刘冰高秀君闫培生张明臣
Owner SHANDONG ANRAN NANOMETRE IND DEV CO LTD
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