New application of potassium iodide or composition containing potassium iodide in preventing or treating African swine fever

An African swine fever and potassium iodide technology, applied in the direction of iodine compound active ingredients, medical preparations containing active ingredients, organic active ingredients, etc. copy effect

Active Publication Date: 2021-07-09
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wherein document (Melchjorsen J, et al.Early Innate Recognition of Herpes Simplex Virus in Human Primary Macrophages Is Mediated via the MDA5 / MAVS-Dependent and MDA5 / MAVS / RNA Polymerase III-Independent Pathways.) discloses RNA polymerase III inhibitor ML60218 There is no inhibition on the levels of interferon IFN-β and TNF-α after HSV-1 infection, that is, ML60218 cannot inhibit the replication of HSV-1 virus

Method used

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  • New application of potassium iodide or composition containing potassium iodide in preventing or treating African swine fever
  • New application of potassium iodide or composition containing potassium iodide in preventing or treating African swine fever
  • New application of potassium iodide or composition containing potassium iodide in preventing or treating African swine fever

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Experimental program
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Embodiment 1

[0034] Effect of embodiment 1KI and ML-60218 on African swine fever virus infection replication and gene transcription expression

[0035] 1. Changes in African swine fever virus infection and replication

[0036] Porcine alveolar macrophages (PAM, 1 × 10 6 / well), the experimental group was treated with KI (100 μM), ML-60218 (50 μM), KI (100 μM) and ML-60218 (50 μM) compositions for 2 hours, and then infected with ASFV CN / GS / 2018 strain (0.1MOI) 24h; Empty cells and empty cells were inoculated with ASFV CN / GS / 2018 strain (0.1MOI) for 24h as a control. Cells and supernatants from different treatments were collected, frozen and thawed three times at -80°C as samples, and serially diluted 10 times with serum-free RPMI 1640 to make 7 dilutions, each dilution repeated 8 wells, and inoculated into PAM Cells were cultured while porcine red blood cells were added; the cell plate was placed at 37°C, 5% CO 2 The conditions were cultured for 5 days, and the hematocyte adsorption react...

Embodiment 2KI

[0047] Example 2 The cytotoxicity of KI combined with ML-60218

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Abstract

The invention belongs to the technical field of African swine fever treatment, and particularly relates to new application of potassium iodide or a composition containing the potassium iodide to prevention or treatment of African swine fever. The invention accidentally finds that potassium iodide (KI) can inhibit the expression of African swine fever virus proteins p72 and p30, inhibit the copy number and virus titer of African swine fever virus genes and inhibit the replication and infection of ASFV, and is used for preventing or treating African swine fever; meanwhile, it is found that potassium iodide (KI) and ML-60218 are combined to remarkably inhibit expression of African swine fever virus proteins p30 and p72, viruses are prevented from invading host cells, and the potassium iodide (KI) and ML-60218 can be used for inhibiting the whole period of ASFV infection; and potassium iodide (KI) and ML-60218 are combined to obviously inhibit the replication of the African swine fever virus and reduce the virus titer after infection of the African swine fever virus, so that the ML-60218 can be used as an inhibitor of the African swine fever virus and is used for preventing or treating the African swine fever.

Description

technical field [0001] The invention belongs to the technical field of treating African swine fever, and in particular relates to a new application of potassium iodide or a composition containing potassium iodide for preventing or treating African swine fever. Background technique [0002] African swine fever (African swine fever, ASF) is caused by the infection of African swine fever virus (ASFV), and is characterized by fever and hemorrhage in pigs. The fatality rate for domestic pigs is as high as 100%. . The disease first broke out in Kenya in 1921 and subsequently became widespread among domestic and wild pigs throughout Africa. It was introduced into Europe in the 1950s, and it took 40 years to eliminate the disease throughout Europe. However, the disease was introduced to Georgia from East Africa again in 2007, and then spread widely in Eastern Europe, and in 2017, it was introduced to Irkutsk in the Russian Far East. In early August 2019, researcher Hu Rongliang t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/18A61P31/20A61K31/4155
CPCA61K33/18A61K31/4155A61P31/20A61K2300/00
Inventor 郑海学李丹吴盼雪李玲霞杨文萍张敬朱国强茹毅田宏秦晓东冯涛杨帆张克山
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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