Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of transposon and its application

A technology of transposons and DNA molecules, applied in the field of transposons and their applications, can solve problems such as no flowering, reduced yield, and abnormal flowering

Active Publication Date: 2022-04-26
CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In North China, the extended corn maturity period will also affect the rotation of the next crop. In Northeast China, the extended corn maturity period will also increase the risk of freezing damage, resulting in reduced production
In addition, maize is domesticated from the tropical plant teosinte, which is sensitive to photoperiod and cannot flower normally under high-latitude long-day conditions. Many tropical maize inbred lines are still photoperiod-sensitive and flower in high-latitude temperate regions The period is very late, even without flowering, which directly restricts the good alleles in tropical maize germplasm resources from being excavated and utilized.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of transposon and its application
  • A kind of transposon and its application
  • A kind of transposon and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1. Discovery of transposon and its application in ZmCCT near-isogenic line

[0068] 1. Discovery of LINE / L1 transposon

[0069] 1. By crossing, combine a very early-maturing maize inbred line F7 from Europe and an F of the normal-maturing maize landrace OGD (OAXACAN GREEN DENT) from Mexico 2 In the group, the difference in the loose powder period of the parents reached one week. Hainan at low latitudes to F 2 QTL mapping was performed in the population, and a major QTL affecting the flowering period of maize was located near 94-Mb of chromosome 10, which could explain 15.3% of the phenotypic variation, with an additive effect of 1.6 days ( figure 1 ).

[0070] 2. Using 5800 individuals from 19 remaining heterozygous families (HIFs) for fine mapping, this QTL was narrowed down to a 520-kb interval by screening for new crossover individuals and molecular markers, located at markers M4 and M7 between( figure 2 ,Table 1).

[0071] Table 1 is the primer seq...

Embodiment 2

[0138] Embodiment 2, the application of LINE / L1 transposon in identifying the flowering time, plant height and ear position of corn to be tested sooner or later

[0139] 1. Extract the genomic DNA of the corn to be tested

[0140] Extract genomic DNA from the maize leaf tissue to be tested.

[0141] The maize to be tested is each family in Table 5, and each family has 22, 32, 28, 36, 44, 32, 72, 63, 69 and 147 strains respectively:

[0142] 1) ft1-1*, ft1-2*, ft2-1*, ft2-2*, ft3-1*, ft3-2* are prepared as follows:

[0143] The parents are F7 and OGD, and the two parents are crossed to obtain F 1 Generation, F 1 Self-bred for 7 generations and got F 7 ;from F 7 Select the F that remains heterozygous for the ZmCCT gene 7 Generation of individual plants (M2 in Table 1 is used for identification, M2 is located on the ZmCCT gene, and 2 heterozygous bands obtained by M2 amplification represent that the ZmCCT gene remains heterozygous), these selected F 7The family composed of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a transposon and its application. The LINE / L1 transposon provided by the present invention is any one of the following: 1) the nucleotide sequence is sequence 5 in the sequence listing; 2) it hybridizes with the DNA sequence defined in 1) under stringent conditions and has the same encoding DNA molecules with functional amino acid residues; 3) at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96% of the DNA sequence defined in 1) %, at least 97%, at least 98%, or at least 99% homologous and encode DNA molecules with identical functional amino acid residues. The present invention discovers the LINE / L1 transposon in the ZmCCT gene for the first time, and finds that the allele ZmCCT-FO containing the transposon can be used to identify the flowering time of the corn to be tested, and can also be used to identify the corn to be tested plant height and ear height.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a transposon and its application. Background technique [0002] Corn is one of the most important food crops in my country. In 2019, corn production was close to 40% of my country's total grain output. Maize maturity is one of the key traits affecting maize yield. Prolonged maize maturity, that is, later flowering of maize, will lead to a higher proportion of energy consumption in the vegetative growth stage and increase the possibility of field encounters with pests and diseases. In North China, prolonged corn maturity will also affect the rotation of the next crop. In Northeast China, prolonged corn maturity will increase the risk of frost damage and reduce yields. In addition, maize is domesticated from the tropical plant ruminant, which is sensitive to photoperiod and cannot bloom normally under high-latitude long-day conditions. Many tropical maize inbred lines are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 林中伟钟舒阳
Owner CHINA AGRI UNIV