A kind of preparation method and application of natural quorum sensing inhibitor
A quorum sensing and inhibitor technology, applied in botany equipment and methods, applications, food ingredients containing natural extracts, etc., can solve problems such as no public reports, achieve rich sources, good application prospects, and facilitate application and promotion Effect
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[0053] Mix rhubarb, Huang Ling, and Cortex Phellodendri in a certain ratio (1:1.5-0.5:0.5-1.5), dry in an oven at 50°C for 24 hours until dry, and use a Retsch high-quality tissue grinder to grind (6.5×1000rpm, 6min). Pass through an 80-mesh sieve to obtain Sanhuang powder; weigh 100g of the powder and add 75% ethanol solution at a ratio of 1:2.5 to liquid, extract at 75°C for 35 minutes, repeat twice, remove the residue by suction filtration and combine the filtrate, add AlCl 3 (6g) and ZnCl 2 (12g) for precipitation, after the solution was separated, centrifuged (3500 r / min, 15 min) after the obvious precipitation appeared, and 200mL, 12% hydrochloric acid was added to the precipitation for transsolution, and the supernatant was obtained by suction filtration under reduced pressure, and added Extract with 2 times the volume of ethyl acetate, concentrate the extract in a rotary evaporator (0.065Mpa, 64°C), and dry the concentrate in an oven at 60°C to obtain the extract of Sa...
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