A kind of preparation method and application of natural quorum sensing inhibitor
A quorum sensing and inhibitor technology, applied in botany equipment and methods, applications, food ingredients containing natural extracts, etc., can solve problems such as no public reports, achieve rich sources, good application prospects, and facilitate application and promotion Effect
Active Publication Date: 2021-11-09
海南达菲医药科技有限公司
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Problems solved by technology
[0006] Sanhuang powder, currently on the market as a veterinary drug, is composed of rhubarb, scutellaria, and cork (5:3:2). As a veterinary drug that is widely used in the field of aquatic product breeding, it has the functions of clearing away heat, detoxifying, antibacterial and anti-inflammatory. It can be used to prevent and treat bacterial diseases such as liver and gallbladder, gill rot, bleeding, enteritis, and rotting skin. At the same time, it can enhance the body's disease-resistant immune function. In the field of aquaculture, Sanhuang powder is often used as feed or directly sprinkled on ponds to prevent and treat fish. disease, but the research and application of sanhuang powder as a spoilage bacteria quorum sensing inhibitor has not been reported publicly so far
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Embodiment 1
[0053] Mix rhubarb, Huang Ling, and Cortex Phellodendri in a certain ratio (1:1.5-0.5:0.5-1.5), dry in an oven at 50°C for 24 hours until dry, and use a Retsch high-quality tissue grinder to grind (6.5×1000rpm, 6min). Pass through an 80-mesh sieve to obtain Sanhuang powder; weigh 100g of the powder and add 75% ethanol solution at a ratio of 1:2.5 to liquid, extract at 75°C for 35 minutes, repeat twice, remove the residue by suction filtration and combine the filtrate, add AlCl 3 (6g) and ZnCl 2 (12g) for precipitation, after the solution was separated, centrifuged (3500 r / min, 15 min) after the obvious precipitation appeared, and 200mL, 12% hydrochloric acid was added to the precipitation for transsolution, and the supernatant was obtained by suction filtration under reduced pressure, and added Extract with 2 times the volume of ethyl acetate, concentrate the extract in a rotary evaporator (0.065Mpa, 64°C), and dry the concentrate in an oven at 60°C to obtain the extract of Sa...
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The invention belongs to the technical field of food storage and preservation, and discloses a preparation method and application of a natural quorum sensing inhibitor. Rhubarb, scutellaria baicalensis and Phellodendron phellodendri are used as raw materials to extract with ethanol aqueous solution to obtain Sanhuang powder extract. After the yellow powder extract is dissolved, filtered and sterilized, the natural quorum sensing inhibitor can be obtained. The three kinds of traditional Chinese medicines used in the present invention are rich in sources, and the preparation process of the extracts is simple and easy to operate. It has been proved for the first time by experiments that the extract of Sanhuang Powder described in the present invention has the activity of inhibiting the quorum sensing system of Violet Bacillus CV026, and can interfere with the spoilage of aquatic products. The quorum sensing system of Bacteria can inhibit the expression of its putrefaction characteristics, and it will not produce drug resistance to bacteria, and is non-toxic and harmless. Prolonging the fresh-keeping time of ova-shaped pomfret can be used for the development and application of natural quorum sensing inhibitors of spoilage bacteria in aquatic products.
Description
technical field [0001] The invention relates to the technical field of food storage and preservation, and more specifically relates to a preparation method and application of a natural quorum sensing inhibitor. Background technique [0002] my country is a big country in the production and consumption of aquatic products. The aquaculture output of aquatic products accounts for 70% of the world's total output. However, the corruption loss of fresh aquatic products is very serious, with an average loss rate of about 15%. Microbial activities are the cause of aquatic product corruption. The main reason is that in the complex microbial flora, only one or several specific microorganisms play a leading role in the spoilage process. At the end of the storage period, these microorganisms have an absolute advantage in number and proportion, called dominant spoilage bacteria or specific microorganisms. Specific spoilage organisms (SSOs), above the freezing temperature, the growth and re...
Claims
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IPC IPC(8): A01N65/36A01P1/00A23B4/20
CPCA01N65/00A01N65/22A01N65/30A01N65/36A23B4/20A23V2002/00A23V2200/10A23V2250/21
Inventor 于淑池周玲玲
Owner 海南达菲医药科技有限公司
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