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Application of rice ostam1 gene in regulation of plant leaf angle

A plant leaf and plant leaf technology is applied in the application field of rice OsTAM1 gene in regulating the angle of plant leaves, which can solve the problem of unclear molecular mechanism, improve catalytic activity, change lignin metabolic flow, and increase cell density. Effect

Active Publication Date: 2022-06-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PAL / TAL gene family is the entrance and is synthesized through a series of enzymatic reactions. At present, there are few reports on PAL / TAL family members, and its molecular mechanism in lignin synthesis is still unclear

Method used

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  • Application of rice ostam1 gene in regulation of plant leaf angle
  • Application of rice ostam1 gene in regulation of plant leaf angle
  • Application of rice ostam1 gene in regulation of plant leaf angle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Construction of plants overexpressing OsTAM1 gene

[0055] 1.1 Overexpression of OsTAM1 gene

[0056] Take the rice leaves of the rice variety Nipponbare (Ni) at the tillering stage, use Takara's RNA extraction reagent and extract total RNA, and use Takara's reverse transcription kit to reverse-transcribe the total RNA into cDNA. Enter the gene accession number (LOC_Os12g33610) of OsTAM1 in rice.plantbiology.msu.edu / ) to obtain the CDs sequence (SEQ ID NO.1) of the gene, and design the synthetic primer pair (33610F: 5′- ATGAGGCTTGCCAAGGA GCA-3'; 33610R: 5'-GCAGCGCTAGCAGATCGTC-3'), after the cDNA of OsTAM gene was obtained by PCR amplification, the target fragment was ligated into pMDC32 vector (pMDC32 vector was purchased from Boyuan Biotechnology Co., Ltd.) obtained), after confirming that the vector was successfully constructed by PCR amplification and sequencing, the constructed overexpression vector OsTAM1-pMDC was introduced into Agrobacterium EHA105 com...

Embodiment 2

[0071] Example 2 The effect of β-Tyr on leaf erection in rice seedling stage

[0072] 2.1 The effect of exogenous addition of β-Tyr on leaf erection in rice seedling stage

[0073] 2.1.1 Take PI (without β-Tyr) seeds for germination in a 30°C incubator, and then sow them in a 96-well plate, such as image 3 In the black box shown in A, a total of 7 plates were sown. After three days of incubation with water, it was replaced with a basic rice culture medium, and β-Tyr (ie 3-amino-3-(4-hydroxyphenyl) was added to the culture medium correspondingly. Propionic acid, molecular formula C 9 H 11 NO 3 ; CAS No.: 6049-54-3) and α-Tyr (2-amino-3-p-hydroxyphenylpropionic acid), so that the final concentrations were 0 μM, 0.05 μM, 0.1 μM, 0.5 μM, of which 0 μM was the control Group (CK), the rest are treatment groups, after culturing for 10 days, the effects of different treatments on leaf angle were observed and the percentage of leaf bending plants on the 10th day was counted. is c...

Embodiment 3

[0087] Example 3 The effect of β-Tyr on the included angle of the flag leaves in the flowering stage of rice

[0088] 3.1 Continue to cultivate the control PI and overexpressed plants (OE-1, OE-2, OE-3) of 2.2 for half a month, and then transplant them to the field for planting, and pay attention to water, fertilizer and pest management during this period. The phenotype of each line was photographed at the flowering stage of rice, and the results were as follows Image 6 and measured the leaf opening angle of rice, using a protractor to measure the sword leaf inclination angle and leaf opening angle of rice (the included angle of rice leaf refers to image 3 C). Three lines of the control and overexpression plants were taken respectively, and each line was replicated 15 times, and the leaf angle and the angle between the leaves of the flag leaves of the rice at the tillering stage were measured for each plant. The statistical results are as follows: Figure 7 A and 7B are sh...

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Abstract

The invention discloses the application of rice OsTAM1 gene in regulating the included angle of plant leaves. In the present invention, it is found that after overexpressing the OsTAM1 gene, the accumulation of 3-amino-3-(4-hydroxyphenyl) propionic acid content in normal rice leaves is promoted, thereby changing the metabolic flow of the phenylpropane metabolic pathway and making the leaf lignin The content of the compound increases, and the density of blade cells increases, thereby increasing the mechanical strength of the blade, making the blade stand upright, and reducing the opening angle of the rice flag leaf; acid, the leaves of rice plants were upright, and the curvature of the leaves at the seedling stage was significantly lower than that of the control. Therefore, the leaf angle of plants can be regulated by overexpressing the OsTAM1 gene or adding exogenous 3-amino-3-(4-hydroxyphenyl) propionic acid, and the variety improvement of plants can also be carried out through molecular breeding, which is helpful Breed compact plant varieties.

Description

technical field [0001] The invention relates to the field of plant genetic engineering and plant metabolism, in particular to the application of rice OsTAM1 gene in regulating the angle of plant leaves. Background technique [0002] Rice (Oryza Sativa) is an important food crop and model plant, and rice is the staple food for more than half of the world's population. In the past two decades, the global arable land area has been greatly reduced, labor costs have increased, and the population has grown rapidly. It is expected that the demand for food will increase significantly in 2050. In addition, with the progress of society and the improvement of people's quality of life, the demand for food is not only required to eat enough, but also to eat well. In order to better alleviate the food crisis caused by the rapid population growth and the continuous reduction of arable land, solving the food problem and satisfying people's quality of life will face stronger challenges. To...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/12A01H6/46C12Q1/6895C12N15/11
CPCC12N9/1096C12N15/8261C12Y206/01C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 颜健白根祥黎平
Owner SOUTH CHINA AGRI UNIV
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