Application of rice OsTAM1 gene in regulation and control of plant leaf included angle

A technology of plant leaves and plant leaves, which is applied in the application field of rice OsTAM1 gene in regulating the angle of plant leaves, can solve the problems such as unclear molecular mechanism, and achieve the effects of improving catalytic activity, increasing mechanical strength, and increasing planting density

Active Publication Date: 2021-07-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PAL / TAL gene family is the entrance and is synthesized through a series of enzymatic reactions. At present, there are few reports on PAL / TAL family members, and its molecular mechanism in lignin synthesis is still unclear

Method used

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  • Application of rice OsTAM1 gene in regulation and control of plant leaf included angle
  • Application of rice OsTAM1 gene in regulation and control of plant leaf included angle
  • Application of rice OsTAM1 gene in regulation and control of plant leaf included angle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The construction of embodiment 1 overexpression OsTAM1 gene plant

[0055] 1.1 Overexpression of OsTAM1 gene

[0056] Take the rice leaves of the rice variety Nipponbare (Ni) at the tillering stage, use Takara’s RNA extraction reagent and extract total RNA, and use Takara’s reverse transcription kit to reverse transcribe the total RNA into cDNA, in the Rice Genome AnnotationProject (http: / / rice.plantbiology.msu.edu / ), input the gene accession number (LOC_Os12g33610) of OsTAM1 to obtain the CDs sequence (SEQ ID NO.1) of the gene, and design a synthetic primer pair (33610F: 5′- ATGAGGCTTGCCAAGGA GCA-3'; 33610R: 5'-GCAGCGCTAGCAGATCGTC-3'), after obtaining the cDNA of the OsTAM gene by PCR amplification, the target fragment was connected to the pMDC32 vector by the method of seamless cloning (the pMDC32 vector was purchased from Boyuan Biological Company Obtained), after confirming that the carrier was successfully constructed by PCR amplification and sequencing, the cons...

Embodiment 2

[0071] The effect of embodiment 2β-Tyr on rice seedling leaf erect

[0072] 2.1 Effect of exogenous addition of β-Tyr on rice seedling leaf erection

[0073] 2.1.1 Take PI (without β-Tyr) seeds to germinate in a 30°C incubator, and then sow them in a 96-well plate, such as image 3 In the black box shown in A, 7 plates were sown together. After adding water and culturing for three days, it was replaced with rice basic culture medium, and β-Tyr (ie 3-amino-3-(4-hydroxyphenyl) Propionic acid, molecular formula C 9 h 11 NO 3 ; CAS No.: 6049-54-3) and α-Tyr (2-amino-3-p-hydroxyphenylpropionic acid), so that the final concentrations were 0 μM, 0.05 μM, 0.1 μM, 0.5 μM, wherein 0 μM was the control group (CK), and the rest are treatment groups. After cultivating for 10 days, observe the influence of different treatments on the leaf angle and count the percentage of the leaf bending number on the 10th day. The leaf curvature refers to the leaf angle greater than 10° is regarded a...

Embodiment 3

[0087] Example 3 Effect of β-Tyr on Flag Leaf Angle at Flowering Stage of Rice

[0088] 3.1 Continue culturing the control PI and overexpression plants (OE-1, OE-2, OE-3) in 2.2 for half a month, and then transplant them into the field for planting. During this period, pay attention to water, fertilizer and pest management. The phenotypes of each line were photographed at the flowering stage of rice, and the results were as follows: Figure 6 shown, and measure the leaf opening angle of rice, the flag leaf inclination angle and leaf opening angle of the paddy rice that adopt protractor to measure (the rice leaf angle refers to image 3 C). Take three lines of control and overexpression plants respectively, each line has 15 repetitions, and each plant measures the flag leaf opening angle and flag leaf angle of 5 rice tillering stages, and the statistical results are as follows: Figure 7 A and 7B are shown. After measuring the leaf angle, the flag leaf samples were freeze-dr...

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Abstract

The invention discloses application of a rice OsTAM1 gene in regulation and control of a plant leaf included angle. It is found that after the OsTAM1 gene is over-expressed, accumulation of the content of 3-amino-3-(4-hydroxyphenyl)propionic acid of normal rice leaves is promoted, so that metabolic flux of a phenylpropane metabolic pathway is changed, the content of lignin of the leaves is increased, the cell density of the leaves is increased, the mechanical strength of the leaves is increased, the leaves stand upright, and the field angle of rice flag leaves is reduced. The invention also finds that by adding the exogenous 3-amino-3-(4-hydroxyphenyl)propionic acid, the leaf of the rice plant is upright, and the leaf bending rate at the seedling stage is obviously reduced compared with that of the control group. Therefore, the leaf included angle of the plant can be regulated and controlled by overexpressing the OsTAM1 gene or adding exogenous 3-amino-3-(4-hydroxyphenyl)propionic acid, the variety of the plant can be improved by molecular breeding, and the breeding of compact plant varieties is facilitated.

Description

technical field [0001] The invention relates to the field of plant genetic engineering and plant metabolism, in particular to the application of rice OsTAM1 gene in regulating plant leaf angle. Background technique [0002] Rice (Oryza Sativa) is an important food crop and model plant, and more than half of the world's population uses rice as a staple food. In the past two decades, the global arable land area has been greatly reduced, labor costs have increased, and the population has grown rapidly. It is expected that the demand for food will increase significantly in 2050. In addition, with the progress of society and the improvement of people's quality of life, people not only need to eat enough food, but also need to eat well. In order to better alleviate the food crisis brought about by rapid population growth and decreasing arable land, solving the food problem and satisfying people's quality of life will face stronger challenges. To solve the food problem, the yield...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/12A01H6/46C12Q1/6895C12N15/11
CPCC12N9/1096C12N15/8261C12Y206/01C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 颜健白根祥黎平
Owner SOUTH CHINA AGRI UNIV
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